海南医学
海南醫學
해남의학
HAINAN MEDICAL JOURNAL
2014年
12期
1717-1719
,共3页
乳腺癌%BRCA1基因表达%启动子区%甲基化
乳腺癌%BRCA1基因錶達%啟動子區%甲基化
유선암%BRCA1기인표체%계동자구%갑기화
Breast cancer%BRCA1 gene expression%The promoter region%Methylation
目的：研究BRCA1基因启动子区甲基化状态在乳腺癌组织中的抑癌作用。方法甲基化PCR法检测60例乳腺癌患者的癌组织、癌旁组织和20例乳腺良性组织中BRCA1-mRNA表达及启动子区甲基化状态，以及评价其作用。结果癌组织和癌旁组织中BRCA1-mRNA的表达水平分别为(0.6122±0.2344)、(0.6875±0.2576)，在癌组织、癌旁组织、良性肿瘤组织中BRCA1表达的甲基化率分别为70.0%、48.3%、35.0%。癌组织的甲基化率明显的高于癌旁组织和良性肿瘤组织；经统计学分析显示，淋巴结转移、肿瘤分级在BRCA1-mRNA表达及启动子区甲基化中的差异均具有统计学意义(P<0.05)，绝经、组织分型在BRCA1-mRNA表达及启动子区甲基化中的差异均无统计学意义(P>0.05)。结论 BRCA1-mRNA表达及启动子区甲基化状态在乳腺癌的发生发展中起着重要的作用，并且与组织分期和淋巴结的转移密切相关。
目的：研究BRCA1基因啟動子區甲基化狀態在乳腺癌組織中的抑癌作用。方法甲基化PCR法檢測60例乳腺癌患者的癌組織、癌徬組織和20例乳腺良性組織中BRCA1-mRNA錶達及啟動子區甲基化狀態，以及評價其作用。結果癌組織和癌徬組織中BRCA1-mRNA的錶達水平分彆為(0.6122±0.2344)、(0.6875±0.2576)，在癌組織、癌徬組織、良性腫瘤組織中BRCA1錶達的甲基化率分彆為70.0%、48.3%、35.0%。癌組織的甲基化率明顯的高于癌徬組織和良性腫瘤組織；經統計學分析顯示，淋巴結轉移、腫瘤分級在BRCA1-mRNA錶達及啟動子區甲基化中的差異均具有統計學意義(P<0.05)，絕經、組織分型在BRCA1-mRNA錶達及啟動子區甲基化中的差異均無統計學意義(P>0.05)。結論 BRCA1-mRNA錶達及啟動子區甲基化狀態在乳腺癌的髮生髮展中起著重要的作用，併且與組織分期和淋巴結的轉移密切相關。
목적：연구BRCA1기인계동자구갑기화상태재유선암조직중적억암작용。방법갑기화PCR법검측60례유선암환자적암조직、암방조직화20례유선량성조직중BRCA1-mRNA표체급계동자구갑기화상태，이급평개기작용。결과암조직화암방조직중BRCA1-mRNA적표체수평분별위(0.6122±0.2344)、(0.6875±0.2576)，재암조직、암방조직、량성종류조직중BRCA1표체적갑기화솔분별위70.0%、48.3%、35.0%。암조직적갑기화솔명현적고우암방조직화량성종류조직；경통계학분석현시，림파결전이、종류분급재BRCA1-mRNA표체급계동자구갑기화중적차이균구유통계학의의(P<0.05)，절경、조직분형재BRCA1-mRNA표체급계동자구갑기화중적차이균무통계학의의(P>0.05)。결론 BRCA1-mRNA표체급계동자구갑기화상태재유선암적발생발전중기착중요적작용，병차여조직분기화림파결적전이밀절상관。
Objective To study the effect of BRCA1 promoter methylation on tumor inhibition in breast can-cer tissue. Methods BRCA1-mRNA expression and promoter methylation were detected by methylation PCR in 60 cases of breast cancer tissue, pericarcinous tissue, and 20 cases of benign mammary gland tissue. Meanwhile, its ef-fect was evaluated. Results The expression of BRCA1-mRNA levels in the cancer tissue and pericarcinous tissue were (0.612 2±0.234 4) and (0.234 4 ±0.257 6), respectively. The methylation rate in cancer tissure, pericarcinous tis-sue and benign tumor tissue were 70.0%, 48.3%and 35.0%, respectively. And the methylation rate in cancer tissue was significantly higher than those in pericarcinous tissue and benign tumor tissue. The differences of lymph node ma-tastasis and tumor classification in BRCA1 mRNA expression and promoter methylation were statistically significant (P<0.05), while there was no difference in menopause and histological stages in BRCA1 mRNA expression and pro-moter region methylation (P>0.05). Conclusion BRCA1 mRNA expression and promoter region methylation status play an important role in the development of breast cancer, and are closely related to the histological stages and lymph node metastasis.
