CAJ | 학술논문

目的：探讨特定浓度（200 mmol/L）高渗钠盐复苏液对严重烫伤大鼠早期肺损伤的影响。方法56只SD大鼠随机分成烫伤组（48只）和对照组（8只），其中烫伤组又分为乳酸钠林格液（LR）组（n＝24）和高渗钠盐液（HS）组（n＝24）。所有大鼠全部颈静脉置管，对照组大鼠不烫伤不补液，对LR组和HS组大鼠进行烫伤后复苏治疗，分别检测各组肺组织的髓过氧化物酶（MPO）与丙二醛（MDA）的含量，将所得数据进行统计学分析；采用免疫组织化学方法测定肺组织p38MAPK的活化程度，所得图片进行比较。结果 HS组复苏后肺组织的MPO含量在注射补液2、8、24h分别为（1．48±0．45）、（1．52±0．31）、（1．46±0．15）U/g与补液2、8、24 h MDA含量（0．80±0．09）、（0．85±0．08）、（0．80±0．12）mmol/mg明显低于对应观测时间点的LR组复苏后肺组织的MPO含量（1．91±0．32）、（1．91±0．32）、（1．79±0．22）U/g 与 MDA 含量（1．06±0．08）、（0．85±0．08）、（0．95±0．06）mmol/mg，差异有统计学意义（P＜0．05）；与对照组的MPO含量（1．49±0．22）U/g与MDA含量（0．78±0．14）mmol/mg比较，差异无统计学意义（P＞0．05）。肺组织的p38MAPK的免疫组化活性检测显示：HS组复苏后2、8、24 h的肺组织p38MAPK的表达与LR组比较明显减少，与对照组比较增加不明显，LR组与对照组比较明显增加。结论严重烫伤大鼠早期使用200 mmol/L高渗盐液进行液体复苏与乳酸钠林格液比较，通过减少肺组织的氧化应激反应与信号转导通路的活化减轻肺损伤。
목적：탐토특정농도（200 mmol/L）고삼납염복소액대엄중탕상대서조기폐손상적영향。방법56지SD대서수궤분성탕상조（48지）화대조조（8지），기중탕상조우분위유산납림격액（LR）조（n＝24）화고삼납염액（HS）조（n＝24）。소유대서전부경정맥치관，대조조대서불탕상불보액，대LR조화HS조대서진행탕상후복소치료，분별검측각조폐조직적수과양화물매（MPO）여병이철（MDA）적함량，장소득수거진행통계학분석；채용면역조직화학방법측정폐조직p38MAPK적활화정도，소득도편진행비교。결과 HS조복소후폐조직적MPO함량재주사보액2、8、24h분별위（1．48±0．45）、（1．52±0．31）、（1．46±0．15）U/g여보액2、8、24 h MDA함량（0．80±0．09）、（0．85±0．08）、（0．80±0．12）mmol/mg명현저우대응관측시간점적LR조복소후폐조직적MPO함량（1．91±0．32）、（1．91±0．32）、（1．79±0．22）U/g 여 MDA 함량（1．06±0．08）、（0．85±0．08）、（0．95±0．06）mmol/mg，차이유통계학의의（P＜0．05）；여대조조적MPO함량（1．49±0．22）U/g여MDA함량（0．78±0．14）mmol/mg비교，차이무통계학의의（P＞0．05）。폐조직적p38MAPK적면역조화활성검측현시：HS조복소후2、8、24 h적폐조직p38MAPK적표체여LR조비교명현감소，여대조조비교증가불명현，LR조여대조조비교명현증가。결론엄중탕상대서조기사용200 mmol/L고삼염액진행액체복소여유산납림격액비교，통과감소폐조직적양화응격반응여신호전도통로적활화감경폐손상。
Objective To explore the effect of 200 mmol/L Na + hypertonic saline resuscitation onpulmonary injury of severely scalded rats in the early stage.Methods Fifty-six SD rats were randomly divided into scald group and control group,and the scald group was divided into sodium lactate Ringer′s solution (LR ) group and hypertonic sodium solution (HS ) group, each group of 24. Neck vein catheterization was used on all rats,and scald resuscitation therapy was adopted in the rats of LR group and HS group.Lung tissue myeloperoxidase (MPO)and lung tissue malondialdehyde (MDA)were examined in three groups.The data drawn from the test were statistically analyzed.The immunohistochemical method was used to determination p38MAPK activation level of lung tissue in the control group and after fluid resuscitationinthegroupLRandgroupHS.Thepictureswerecompared.Results Thepulmonarytissue MPO levels of the group HS at 2 h (1.48 ±0.45),8 h (1.52 ±0.31)and 24 h (1.46 ±0.15)U/g after resuscitation were significantly lower than those of the group LR at 2 h (1.91 ±0.32),8 h (1.91 ± 0.32)and 24 h (1.79 ±0.22)U/g respectively,and the pulmonary tissue MDA levels of the group HS at 2 h (0.80 ±0.09 ),8 h (0.85 ±0.08 )and 24 h (0.80 ±0.12 )mmol/mg after resuscitation were significantly lower than those of the group LR at 2 h (1.06 ±0.08),8 h (0.85 ±0.08)and 24 h (0.95 ± 0.06)mmol/mg respectively,the difference was statistically significant (P <0.05 or P <0.01 ).But compared with the control group on MPO level (1.49 ±0.22)U/g and MDA level (0.78 ± 0.14)mmol/mg,the pulmonary tissue MPO and MDA levels of the group HS had no significant difference (P>0.05).The p38MAPK activity contents of lung tissue by immunohistochemical assay in the group HS at 2 h,8 h,24 h after fluid resuscitation were significantly expressed in comparison with the group LR,and had no obviously difference with the control group.The p38MAPK activity contents of lung tissue in the group LR at 2 h,8 h and 24 h after resuscitation increased significantly expression compared with the control group.Conclusions ComparedwithLR,supplementingHScanattenuateoxidativestressandp38MAPK activity contents of lung tissue significantly,which ameliorate pulmonary injury of severely scalded rats in the early stage.