中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
1期
56-58,61
,共4页
王忠超%李跃华%张彬%刘兰涛%魏成喜%额都%任立群
王忠超%李躍華%張彬%劉蘭濤%魏成喜%額都%任立群
왕충초%리약화%장빈%류란도%위성희%액도%임립군
蒙药乳腺-I号%乳腺增生%细胞凋亡
矇藥乳腺-I號%乳腺增生%細胞凋亡
몽약유선-I호%유선증생%세포조망
Mongolian Remedy RuXian-I%hyperplasia of mammary glands%apoptosis
目的:研究蒙药乳腺-I(M-I)号对乳腺增生大鼠乳腺组织细胞增殖和凋亡的影响。方法实验动物采用雌性未孕Wistar大鼠,乳腺增生模型复制方法为大鼠肌肉注射苯甲酸雌二醇0.5 mg/(kg·d)25 d,随后肌肉注射黄体酮4 mg/(kg·d)5 d。实验共分为6组:正常对照组、模型对照组,均给与生理盐水灌胃;阳性药对照组,给与三苯氧胺1.8 mg/kg灌胃;M-I号低、中和高剂量组分别给予M-I号0.5 g/kg、1.0 g/kg和3.0 g/kg灌胃。给药结束后,通过对各组大鼠乳腺组织进行HE染色,观察大鼠乳腺组织增生的程度,应用免疫组织化学方法观察乳腺组织中增殖细胞核抗原、细胞凋亡相关调控因子Bcl-2、Bax的表达。结果 M-I号能够抑制乳腺组织增生,降低PCNA和Bcl-2的表达活性(P<0.05),增加Bax的表达活性(P<0.05),进而抑制乳腺组织细胞增殖,促进细胞凋亡(P<0.05)。结论 M-I号对乳腺增生大鼠有较好的治疗作用,其治疗机制与抑制大鼠乳腺组织细胞增殖,促进细胞凋亡的作用有关。
目的:研究矇藥乳腺-I(M-I)號對乳腺增生大鼠乳腺組織細胞增殖和凋亡的影響。方法實驗動物採用雌性未孕Wistar大鼠,乳腺增生模型複製方法為大鼠肌肉註射苯甲痠雌二醇0.5 mg/(kg·d)25 d,隨後肌肉註射黃體酮4 mg/(kg·d)5 d。實驗共分為6組:正常對照組、模型對照組,均給與生理鹽水灌胃;暘性藥對照組,給與三苯氧胺1.8 mg/kg灌胃;M-I號低、中和高劑量組分彆給予M-I號0.5 g/kg、1.0 g/kg和3.0 g/kg灌胃。給藥結束後,通過對各組大鼠乳腺組織進行HE染色,觀察大鼠乳腺組織增生的程度,應用免疫組織化學方法觀察乳腺組織中增殖細胞覈抗原、細胞凋亡相關調控因子Bcl-2、Bax的錶達。結果 M-I號能夠抑製乳腺組織增生,降低PCNA和Bcl-2的錶達活性(P<0.05),增加Bax的錶達活性(P<0.05),進而抑製乳腺組織細胞增殖,促進細胞凋亡(P<0.05)。結論 M-I號對乳腺增生大鼠有較好的治療作用,其治療機製與抑製大鼠乳腺組織細胞增殖,促進細胞凋亡的作用有關。
목적:연구몽약유선-I(M-I)호대유선증생대서유선조직세포증식화조망적영향。방법실험동물채용자성미잉Wistar대서,유선증생모형복제방법위대서기육주사분갑산자이순0.5 mg/(kg·d)25 d,수후기육주사황체동4 mg/(kg·d)5 d。실험공분위6조:정상대조조、모형대조조,균급여생리염수관위;양성약대조조,급여삼분양알1.8 mg/kg관위;M-I호저、중화고제량조분별급여M-I호0.5 g/kg、1.0 g/kg화3.0 g/kg관위。급약결속후,통과대각조대서유선조직진행HE염색,관찰대서유선조직증생적정도,응용면역조직화학방법관찰유선조직중증식세포핵항원、세포조망상관조공인자Bcl-2、Bax적표체。결과 M-I호능구억제유선조직증생,강저PCNA화Bcl-2적표체활성(P<0.05),증가Bax적표체활성(P<0.05),진이억제유선조직세포증식,촉진세포조망(P<0.05)。결론 M-I호대유선증생대서유교호적치료작용,기치료궤제여억제대서유선조직세포증식,촉진세포조망적작용유관。
Objective To observe effects of Mongolian Remedy RuXian-I on proliferation and apoptosis of Mammary Gland Hyperplastic Tissue in Rats. Method 8 rats randomly chosen from Forty-eight virgin female Wistar rats were regarded as normal. The others were injected intramuscular with E 2(0.5 mg/kg) per day for 25 d and P(4 mg/kg) per day for 5 d. The rats in normal control group were injected with normal saline. Then disease model group was randomly divided into 5 groups:model control group, positive control group, M-I low dosage group, M-I middle dosage group and M-I high dosage group. The rats in different groups were respectively treated with normal saline, tamoxifen, M-I 0.5, 1.0 and 3.0 g/kg for 28 d. Then the pathological changes and expressions of Bax, Bcl-2, PCNA in breast tissues were measured. Results Compared with normal control group, expression of PCNA and Bcl-2 in model control group were obviously higher(P<0.01, P<0.05), while the expression of Bax were obviously lower (P<0.01). Compared with model control group, the expressions of PCNA and Bcl-2 in breast tissue in M-I high dosage group were obviously lower, while the expressions of Bax breast tissues were obviously higher(P<0.05). Conclusion Treatment mechanism of M-I may be related with inhibited cell proliferation and promote cell apoptosis in the breast tissue.