CAJ | 학술논문

目的：研究卡托普利预处理对急性心肌损伤大鼠心肌细胞凋亡及gp 130表达的影响及意义。方法将60只Wistar雄性大白鼠随机分为3组：异丙肾上腺素损伤组、卡托普利预处理组、对照组（n=20）；用TUNEL方法检测心肌细胞凋亡；用免疫组化方法检测Bcl-2和Bax蛋白表达；用Western blot方法检测心肌组织gp 130表达。结果卡托普利预处理组与异丙肾上腺素损伤组比较，心肌细胞凋亡指数降低，Bax、gp 130表达减少，Bcl-2表达增加（P<0.01或P<0.05）。结论卡托普利预处理可通过降低gp 130的表达来减少急性心肌损伤引起的细胞凋亡。
목적：연구잡탁보리예처리대급성심기손상대서심기세포조망급gp 130표체적영향급의의。방법장60지Wistar웅성대백서수궤분위3조：이병신상선소손상조、잡탁보리예처리조、대조조（n=20）；용TUNEL방법검측심기세포조망；용면역조화방법검측Bcl-2화Bax단백표체；용Western blot방법검측심기조직gp 130표체。결과잡탁보리예처리조여이병신상선소손상조비교，심기세포조망지수강저，Bax、gp 130표체감소，Bcl-2표체증가（P<0.01혹P<0.05）。결론잡탁보리예처리가통과강저gp 130적표체래감소급성심기손상인기적세포조망。
Objective To study the influence of Captopril preconditioning on apoptosis and the expression of gp 130 in rats of acute myocardial injured. Method Wistar rats were randomly divided into three groups:control group, Iso group and Cap preconditioning group. Cardiomyocytes apoptosis was detected with TUNEL method. The expressions of Bcl-2 and Bax proteins in myocardium were tested by immunohistochemistry. The expression of gp 130 was tested with Western blot method. Results The index of cardiomyocytes apoptosis was decreased, the expressions of Bax proteins and gp 130 were decreased and the expression of Bcl-2 proteins was increased in Cap preconditioning group compared with Iso group (P<0.01 or P<0.05). Conclusion The expression of gp 130 could be decreased through Cap preconditioning, which can reduce the cardiomyocytes apoptosis.