CAJ | 학술논문

目的：探讨灯盏细辛注射液对缺血-再灌注肾脏损伤的保护作用，为临床防治肾缺血-再灌注损伤提供理论依据。方法36只SPF级健康雄性SD大鼠，随机均分为3组（n=12）。A组：缺血-再灌注组；B组：灯盏细辛注射液预处理组；C组：假手术组。A、C组大鼠腹腔注射生理盐水，B组大鼠腹腔注射灯盏细辛注射液12 mg/kg。14天后比较3组大鼠肾功能指标、肾抗氧化指标、肾组织细胞凋亡指数以及肾组织Bcl-2、Bax表达情况。结果 B组血尿素氮（blood urea nitrogen，BUN）和血肌酐(serum creatinine，SCr)显著低于A组（P<0.05），且显著高于C组（P<0.05）；同时，B组超氧岐化酶（superoxide dismutase，SOD）显著高于A组（P<0.05）而显著低于C组（P<0.05），B组丙二醛（malondialdehyde，MDA）显著低于A组（P<0.05）而显著高于C组（P<0.05）；肾组织细胞凋亡指数结果显示：B组细胞凋亡指数AI极显著低于A组（P<0.01）而显著高于C组（P<0.01）；肾组织Bcl-2、Bax免疫组化染色结果显示：B组Bcl-2表达显著高于A组与C组（P<0.01），B组Bax表达显著低于A组（P<0.05）而显著高于C组（P<0.01），B组Bcl-2/Bax值显著高于A组与C组（P<0.05）。结论灯盏细辛注射液可能通过抗氧化和抗凋亡作用对肾脏缺血-再灌注损伤起到保护作用。
목적：탐토등잔세신주사액대결혈-재관주신장손상적보호작용，위림상방치신결혈-재관주손상제공이론의거。방법36지SPF급건강웅성SD대서，수궤균분위3조（n=12）。A조：결혈-재관주조；B조：등잔세신주사액예처리조；C조：가수술조。A、C조대서복강주사생리염수，B조대서복강주사등잔세신주사액12 mg/kg。14천후비교3조대서신공능지표、신항양화지표、신조직세포조망지수이급신조직Bcl-2、Bax표체정황。결과 B조혈뇨소담（blood urea nitrogen，BUN）화혈기항(serum creatinine，SCr)현저저우A조（P<0.05），차현저고우C조（P<0.05）；동시，B조초양기화매（superoxide dismutase，SOD）현저고우A조（P<0.05）이현저저우C조（P<0.05），B조병이철（malondialdehyde，MDA）현저저우A조（P<0.05）이현저고우C조（P<0.05）；신조직세포조망지수결과현시：B조세포조망지수AI겁현저저우A조（P<0.01）이현저고우C조（P<0.01）；신조직Bcl-2、Bax면역조화염색결과현시：B조Bcl-2표체현저고우A조여C조（P<0.01），B조Bax표체현저저우A조（P<0.05）이현저고우C조（P<0.01），B조Bcl-2/Bax치현저고우A조여C조（P<0.05）。결론등잔세신주사액가능통과항양화화항조망작용대신장결혈-재관주손상기도보호작용。
Objective To investigate the protective effect of breviscapine on ischemia-reperfusion renal injury, which provides scientific theoretical basis for clinical prevention and treatment of renal ischemia-reperfusion injury. Method 36 SPF male healthy SD rats were randomly divided into 3 groups, 12 rats in each group. Group A was ischemia-reperfusion group, group B was erigeron breviscapus injection preconditioning group, and group C was sham operation group. Rats in group A and C were injected with normal saline, while rats in group B were given 12 ml/kg erigeron breviscapus injection by intraperitoneal injection., After 14 days, renal function, renal antioxidant indexes, renal cell apoptosis indexes and expression of Bcl-2, Bax in renal tissue of three groups were compared. Results The indexes of renal function showed that blood urea nitrogen (BUN) and Serum creatinine (SCr) in group B were significantly lower than group A (P<0.05), but significantly higher than group C (P<0.05). Renal antioxidant indexes showed that superoxide dismutase (SOD) and malondialdehyde (MDA) in group B were significantly higher than group A (P<0.05), but significantly lower than group C(P<0.05). The index of renal cell apoptosis showed that the apoptosis index AI in group B was significantly lower than group A (P<0.01), but significantly higher than group C (P<0.01). The results of immunohistochemical staining showed that the expression of Bcl-2 in group B were significantly higher than that of group A and group C (P<0.05), and the expression of Bax in group B was significantly lower than that in group A (P<0.05) while significantly higher than group C (P<0.01). The ratio of Bcl-2/Bax in group B was significantly higher than group A and group C (P<0.05). Conclusion Erigeron Breviscapus Injection may have a protective role on renal ischemia reperfusion injury though antioxidant and anti-apoptosis.