CAJ | 학술논문

目的：通过超声照射纳米多聚体实验，了解超声对纳米多聚体靶向可控释放DNA的影响。方法实验用溶剂蒸干法制备纳米多聚体--聚乳酸/乙醇酸共聚物（Polylactic/poly glycolic acid， PLGA），为表面携带雄激素受体PLGA纳米颗粒，其包裹有编码荧光蛋白（GFP）的DNA。配制PLGA溶液2 h后，用2种超声方式，不同占空比及不同时间辐照后将溶液离心，定量观察DNA释放情况及细胞荧光表达效果。结果超声能破坏PLGA纳米壳，并释放DNA。辐照组的多聚体DNA释放量均高于对照组；DNA释放量随超声辐照的声强、时间增加变化不显著；连续超声波辐照降解作用略强于脉冲波；胰淀粉酶对DNA释放影响不大。结论体外实验证实超声有促进PLGA纳米多聚体降解及DNA释放的作用。
목적：통과초성조사납미다취체실험，료해초성대납미다취체파향가공석방DNA적영향。방법실험용용제증간법제비납미다취체--취유산/을순산공취물（Polylactic/poly glycolic acid， PLGA），위표면휴대웅격소수체PLGA납미과립，기포과유편마형광단백（GFP）적DNA。배제PLGA용액2 h후，용2충초성방식，불동점공비급불동시간복조후장용액리심，정량관찰DNA석방정황급세포형광표체효과。결과초성능파배PLGA납미각，병석방DNA。복조조적다취체DNA석방량균고우대조조；DNA석방량수초성복조적성강、시간증가변화불현저；련속초성파복조강해작용략강우맥충파；이정분매대DNA석방영향불대。결론체외실험증실초성유촉진PLGA납미다취체강해급DNA석방적작용。
Objective To identify the effective results of ultrasound in degradation of polymeric nanoparticles released DNA .Polymeric nanoparticles was made by dehydration of polyacetylglutamicacid (PLGA, polylactic-co-glycolic acid)solution. Method Green Fluorescent Protein (GFP) was enclosed by PLGA. Different kinds of ultrasound mode and different duct cycle and power ones were used to radiate PLGA solution for 90 s, 9 min, 20 min separately after the solution prepared for 2 hrs,then putted the solution on centrifugal machine at 13000 r/m. Using Choloroform to get rid of fat-soluble impurity,then applied nanodrop to survey the releasing rate of DNA. Finally the effect of cell expression were observed by fluorescent microscope. Results The amount of DNA released from PLGA in groups which were exposed to ultrasound were significantly different from the groups which were not exposed to ultrosound. The releasing amount of former groups had upper limitation. The releasing rate was increased with the increment of the irradiation time,frequency of ultrasound;The effect of the DNA releasing and PLGA degradation by continuous-wave irradiation was stronger than pulsed-wave ultrasound. Conclusion Ultrasound can promote the degradation of PLGA, and do help in DNA releasing and expression in vitro.