CAJ | 학술논문

目的：制备转铁蛋白修饰共载细胞毒药物阿霉素(doxorubicin，DOX)和抗新生血管药物人参皂甙Rg3脂质体（TF modified doxorubicin and Rg 3 loaded liposome,TF-LP-DOX/Rg 3），研究与胃癌细胞的亲和力以及对胃癌细胞的增殖抑制作用。方法采用后插入法制备转铁蛋白修饰共载阿霉素和人参皂甙Rg3脂质体（TF-LP-DOX/Rg 3）。通过定量的细胞摄取实验和定性共聚焦实验研究胃癌细胞对普通脂质体（liposome,LP）和转铁蛋白修饰脂质体（TF modified liposome,TFLP）的亲和力。MTT实验研究不同载药脂质体对胃癌细胞的增殖抑制能力。构建MKN-28胃癌细胞肿瘤球模型，研究不同脂质体对肿瘤球的穿透能力和肿瘤球生长抑制作用。结果细胞摄取实验结果显示，MKN-28细胞对TF-LP的摄取效率是LP的2.9倍（P<0.01）；MTT实验表明,TF-LP-DOX/Rg3对MKN-28细胞的增殖抑制能力显著强于其他组(P<0.01)；肿瘤球实验结果显示，TF-LP-DOX/Rg3具有良好的肿瘤球穿透作用和肿瘤球生长抑制作用。结论转铁蛋白修饰共载细胞毒药物阿霉素(DOX)和抗新生血管药物人参皂甙Rg3脂质体是一种潜在高效的胃癌靶向给药系统。
목적：제비전철단백수식공재세포독약물아매소(doxorubicin，DOX)화항신생혈관약물인삼조대Rg3지질체（TF modified doxorubicin and Rg 3 loaded liposome,TF-LP-DOX/Rg 3），연구여위암세포적친화력이급대위암세포적증식억제작용。방법채용후삽입법제비전철단백수식공재아매소화인삼조대Rg3지질체（TF-LP-DOX/Rg 3）。통과정량적세포섭취실험화정성공취초실험연구위암세포대보통지질체（liposome,LP）화전철단백수식지질체（TF modified liposome,TFLP）적친화력。MTT실험연구불동재약지질체대위암세포적증식억제능력。구건MKN-28위암세포종류구모형，연구불동지질체대종류구적천투능력화종류구생장억제작용。결과세포섭취실험결과현시，MKN-28세포대TF-LP적섭취효솔시LP적2.9배（P<0.01）；MTT실험표명,TF-LP-DOX/Rg3대MKN-28세포적증식억제능력현저강우기타조(P<0.01)；종류구실험결과현시，TF-LP-DOX/Rg3구유량호적종류구천투작용화종류구생장억제작용。결론전철단백수식공재세포독약물아매소(DOX)화항신생혈관약물인삼조대Rg3지질체시일충잠재고효적위암파향급약계통。
Objective To prepare TF conjugated doxorubicin and Rg 3 loaded liposome and evaluate their properties and effect on the treatment of gastric cancer. Method The liposomes were prepared by thin iflm hydration method. The efifciency of cellular uptake on MKN-28 cells in vitro was evaluated. The anti-proliferation efifciency of TF-LP-DOX/Rg 3 was evaluated by MTT assay. Tumor spheroids were used to evaluate anti-tumor ability of TF-LP-DOX/Rg 3. Results The result demonstrated that TF-LP uptaken by MKN-28 were 2.9 times higher than that of LP(P<0.01). The MTT assay and the inhibition of tumor spheroids in vitro conifrmed strong inhibitory effect of TF-LP-DOX/Rg 3. Conclusion TF-LP-DOX/Rg 3 easily prepared and it is a potential delivery system for the treatment of gastric cancer.