CAJ | 학술논문

目的：探讨锌指转录因子Snail在喉鳞状细胞癌中的表达情况及其与临床特征的关系,通过检测不同喉癌细胞亚群中Snail的表达研究其与喉癌侵袭转移的相关性。方法免疫组化法检测吉林大学第二医院耳鼻咽喉头颈外科48例喉癌组织和20例癌旁对照组织手术标本Snail蛋白的表达情况，并分析Snail与临床特征的相关性。采用RT-PCR及Western blot和细胞免疫化学染色检测CD 44+CD 133+、CD 44-和CD 133-不同亚群喉癌细胞中Snail和粘附分子E-cadherin的表达情况，并与侵袭及转移进行相关性探讨。结果 Snail在喉癌组织中表达水平显著高于癌旁对照组织(P＜0.01)。Snail的表达与喉癌的临床分期、淋巴结转移、肿瘤分化程度密切相关（P＜0.05），而与患者的性别、年龄、临床分型等无关。RT-PCR及Western blot检测结果均证实，Snail在CD 44+CD 133+喉癌细胞中的表达水平明显高于CD 44-和CD 1333-喉癌细胞，而粘附分子E-cadherin在CD 44+CD 133+喉癌细胞中的表达水平明显低于CD 44-和CD 133-喉癌细胞，细胞免疫组化染色证实CD 44+CD 133+喉癌亚群细胞中Snail和E-cadherin的表达呈负相关。结论 Snail的表达与喉癌的侵袭转移密切相关,且在高致瘤亚群CD 44+CD 133+喉癌细胞中呈显著高表达，与粘附分子E-cadherin的表达呈负相关，与喉癌细胞的侵袭和转移具有密切相关性。
목적：탐토자지전록인자Snail재후린상세포암중적표체정황급기여림상특정적관계,통과검측불동후암세포아군중Snail적표체연구기여후암침습전이적상관성。방법면역조화법검측길림대학제이의원이비인후두경외과48례후암조직화20례암방대조조직수술표본Snail단백적표체정황，병분석Snail여림상특정적상관성。채용RT-PCR급Western blot화세포면역화학염색검측CD 44+CD 133+、CD 44-화CD 133-불동아군후암세포중Snail화점부분자E-cadherin적표체정황，병여침습급전이진행상관성탐토。결과 Snail재후암조직중표체수평현저고우암방대조조직(P＜0.01)。Snail적표체여후암적림상분기、림파결전이、종류분화정도밀절상관（P＜0.05），이여환자적성별、년령、림상분형등무관。RT-PCR급Western blot검측결과균증실，Snail재CD 44+CD 133+후암세포중적표체수평명현고우CD 44-화CD 1333-후암세포，이점부분자E-cadherin재CD 44+CD 133+후암세포중적표체수평명현저우CD 44-화CD 133-후암세포，세포면역조화염색증실CD 44+CD 133+후암아군세포중Snail화E-cadherin적표체정부상관。결론 Snail적표체여후암적침습전이밀절상관,차재고치류아군CD 44+CD 133+후암세포중정현저고표체，여점부분자E-cadherin적표체정부상관，여후암세포적침습화전이구유밀절상관성。
Objective To study the expression of Snail in laryngeal carcinoma and to explore its relationship with invasion and metastasis in subgroup laryngeal carcinoma cells. Method The expression of Snail protein was examined by immunohistochemistry staining in the tissue of laryngeal carcinoma. The statistical evaluation was performed to detect the correlation between the Snail protein expression and clinical features. In different subgroups of laryngeal carcinoma cells (CD 44+CD 133+cells, CD 44 and CD 133-cells), the expression of Snail and adhesion molecules E-cadherin were detected by RT-PCR,Western blot test and immunochemical staining which were studied in the correlation with invasion and metastasis. Results The result of immunohistochemical staining revealed that Snail was moderately or highly expressed in the tissue of laryngeal carcinoma significantly and higher than those in adjacent non-cancerous tissues(P<0.01). Expression of Snail was highly correlated with lymph node metastasis, tumor differentiation and clinical classification(P<0.05).However, it was not related to the age , gender and clinical type. RT-PCR and Western blot test results confirmed that the expression level of Snail was significantly higher in CD 44+CD 133+laryngeal cancer cells than in CD 44-and CD 133-laryngeal cancer cells, otherwise the expression level of E-cadherin in CD 44+CD 133+laryngeal cancer cells was significantly lower than in the CD 44-and CD 133-laryngeal cancer cells. Cell immunohistochemical staining confirmed the expression of Snail and E-cadherin were negatively correlated in CD 44+CD 133+laryngeal cancer cells. Conclusion Over expression of Snail in laryngeal carcinoma is closely related to the development of laryngeal cancer and lymph node metastasis. The expression of Snail in the CD 44+CD 133+laryngeal cancer cell subgroup is negatively correlated with adhesion molecules E-cadherin, which is a close correlation with invasion and metastasis of laryngeal cancer cells.