CAJ | 학술논문

照组相比，随着大鼠含药血清浓度的升高，心肌细胞存活率下降（ P＜0．05）。给药组LDH含量显著升高（ P＜0．05）；Na＋-K＋-ATP酶活力随着浓度升高而降低（P＜0．05）；Na＋-K＋-ATP酶含量明显降低（P＜0．05），血清浓度在8％，16％时，细胞凋亡率明显升高（P＜0．05）。结论：草乌对心肌细胞有明显毒害作用，与瓜蒌、白及配伍后毒性增强；其增毒的机制是能显著增大心肌细胞膜通透性，破坏细胞能量系统，诱导细胞自身凋亡，加速细胞死亡。
조조상비，수착대서함약혈청농도적승고，심기세포존활솔하강（ P＜0．05）。급약조LDH함량현저승고（ P＜0．05）；Na＋-K＋-ATP매활력수착농도승고이강저（P＜0．05）；Na＋-K＋-ATP매함량명현강저（P＜0．05），혈청농도재8％，16％시，세포조망솔명현승고（P＜0．05）。결론：초오대심기세포유명현독해작용，여과루、백급배오후독성증강；기증독적궤제시능현저증대심기세포막통투성，파배세포능량계통，유도세포자신조망，가속세포사망。
Objective:To study the toxic effect and mechanism on rat's myocardial cell of Radix Aconiti Kusnezoffii and the Compatibility of Radix Aconiti Kusnezoffii with Fructus Trichosanthis ,Bletillae Rhizoma .Methods:based on vitro myocardial cell culture , it was giv-en with serum with 2%,8%,16%drugs,dertermined myocardial cell vitality by MTT for comparing the toxicity to myocardial cell . Then determine the content of LDH ,vitality of Na +-K+-ATP enzyme,content of Na +-K+-ATP enzyme,cell apoptosis rate by re-spectively kits to research the mechanism .Results:compared with control group , with the content of drugs in serum rise , myocardial cell vitality decline(P<0.05);the content of LDH in given group rise obviously (P<0.05);the vitality of Na +-K+-ATP enzyme of given groups decline (P<0.05);the content of Na +-K+-ATP enzyme decline obviously (P<0.05);cell apoptosis rate of given groups rise obviously as serum with 8%,16%drugs (P<0.05).Conclusion:Aconiti Kusnezoffii Radix have strong harm for cell , and after compatibility with anti-drug, toxicity is more stronger than itself .The toxicity increasing mechanism is knowed that Aconiti Kus-nezoffii Radix can enlarge permeability of myocardial cytomembrane , destroy energy system of cell , induce cell apoptotic , which finally accelerate the death of myocardial cell .