CAJ | 학술논문

目的：观察热敏灸对失眠大鼠血清和脑干超氧化物歧化酶（ SOD）、丙二醛（ MDA）的影响，了解热敏灸治疗失眠的机制。方法：雄性SD大鼠32只，随机分为4组：正常对照组、模型组、艾灸组和热敏灸组，每组8只。采用腹腔注射对氯苯丙氨酸（ PCPA）制备失眠大鼠模型；用Ethovision XT视频跟踪系统录制大鼠活动、观察大鼠的行为学表现；用Morris水迷宫测试大鼠的学习记忆力；用分光光度计检测SOD活性和MDA含量。结果：与正常组相比，模型组大鼠昼夜节律消失；逃避潜伏期延长；SOD活性降低，而MDA含量增加。与模型组相比，热敏灸组大鼠昼夜活动节律基本恢复正常；逃避潜伏期缩短；SOD活性增高，而MDA含量下降。结论：热敏灸具有缓解失眠大鼠症状的作用，可能与其提高机体SOD活性、降低MDA含量有关；热敏灸的效果在某种程度上优于传统悬灸。
목적：관찰열민구대실면대서혈청화뇌간초양화물기화매（ SOD）、병이철（ MDA）적영향，료해열민구치료실면적궤제。방법：웅성SD대서32지，수궤분위4조：정상대조조、모형조、애구조화열민구조，매조8지。채용복강주사대록분병안산（ PCPA）제비실면대서모형；용Ethovision XT시빈근종계통록제대서활동、관찰대서적행위학표현；용Morris수미궁측시대서적학습기억력；용분광광도계검측SOD활성화MDA함량。결과：여정상조상비，모형조대서주야절률소실；도피잠복기연장；SOD활성강저，이MDA함량증가。여모형조상비，열민구조대서주야활동절률기본회복정상；도피잠복기축단；SOD활성증고，이MDA함량하강。결론：열민구구유완해실면대서증상적작용，가능여기제고궤체SOD활성、강저MDA함량유관；열민구적효과재모충정도상우우전통현구。
Objective:To observe the effect of heat -sensitive moxibustion on the activity of superoxide dismutase ( SOD) and the con-tent of malondialdehyde ( MDA) in the serum and brain stem of insomnia rats .Methods:32 male SD rats were divided randomly into 4 groups with 8 rats in each group , i.e.normal control group , model group , moxibustion group , heat-sensitive moxibustion group .The model was induced by para -chlorophenylalanine ( PCPA) intraperitoneal injection .The rats'behavior was regularly recorded half -hour per day with Ethovision XT video tracking system .Learning and memory abilities were evaluated with Morris water maze .SOD ac-tivity and MDA content were determined with spectrophotometer .Results:Compared with rats in normal group , model group rats showed that the sleep-wake rhythm disappeared , the escape period prolonged , and the activity of SOD reduced while the content of MDA increased.Compared with rats in model group , heat-sensitive moxibustion group rats revealed that insomnia symptoms gradually restored, the escape period shortened , and the activity of SOD increased while the content of MDA decreased .Conclusion:Heat-sen-sitive moxibustion improves insomnia symptoms , which might be related to improving antioxidative capacity .