中国农村卫生
中國農村衛生
중국농촌위생
CHINA RURAL HEALTH
2014年
z2期
74-76
,共3页
三氯乙烯%L02肝细胞%凋亡基因%细胞色素P450
三氯乙烯%L02肝細胞%凋亡基因%細胞色素P450
삼록을희%L02간세포%조망기인%세포색소P450
Trichloroethylene%L02 cells%apoptosis genes%cytochrome P450
目的:研究三氯乙烯(TCE)对人肝细胞(L02细胞)的凋亡基因(Caspase -3、Caspase-8、BAX、Bcl -2)和CYP基因(CYP1A2、CYP3A4、CYP2E1) mRNA表达水平的影响。方法:用含10%胎牛血清的RPMI-1640培养肝细胞,DMSO作为溶剂对照,采用不同浓度TCE(0.2、0.4、0.8、1.6、3.2 mmol/L)染毒L02细胞24 h ,另外用TCE 1.0 mmol/L染毒细胞不同时间(2h ,4h ,8h ,16h ,32h),采用Real-time荧光定量PCR技术检测肝细胞中凋亡基因和CYP基因mRNA表达水平。结果:不同剂量TCE染毒后,凋亡基因Caspase-3、Caspase-8、BAX表达水平比对照组升高21%~145%,差异有统计学意义(P<0.05或P<0.01),Bcl-2表达水平下降20%~35%。TCE染毒引起CYP1A2、CYP3A4、CYP2E1的 mRNA表达明显上升,比对照组升高30%~550%,差异有统计学意义(P<0.05或P<0.01)。用TCE 1.0 mmol/L染毒2 h~32 h ,同样发现凋亡基因和CYP表达增加,与对照组比较差异有统计学意义(P<0.05,或P<0.01)。结论:TCE可引起L02肝细胞中凋亡基因和CYP基因表达变化,凋亡基因和CYP基因可能在TCE对肝细胞毒性效应中发挥重要作用。
目的:研究三氯乙烯(TCE)對人肝細胞(L02細胞)的凋亡基因(Caspase -3、Caspase-8、BAX、Bcl -2)和CYP基因(CYP1A2、CYP3A4、CYP2E1) mRNA錶達水平的影響。方法:用含10%胎牛血清的RPMI-1640培養肝細胞,DMSO作為溶劑對照,採用不同濃度TCE(0.2、0.4、0.8、1.6、3.2 mmol/L)染毒L02細胞24 h ,另外用TCE 1.0 mmol/L染毒細胞不同時間(2h ,4h ,8h ,16h ,32h),採用Real-time熒光定量PCR技術檢測肝細胞中凋亡基因和CYP基因mRNA錶達水平。結果:不同劑量TCE染毒後,凋亡基因Caspase-3、Caspase-8、BAX錶達水平比對照組升高21%~145%,差異有統計學意義(P<0.05或P<0.01),Bcl-2錶達水平下降20%~35%。TCE染毒引起CYP1A2、CYP3A4、CYP2E1的 mRNA錶達明顯上升,比對照組升高30%~550%,差異有統計學意義(P<0.05或P<0.01)。用TCE 1.0 mmol/L染毒2 h~32 h ,同樣髮現凋亡基因和CYP錶達增加,與對照組比較差異有統計學意義(P<0.05,或P<0.01)。結論:TCE可引起L02肝細胞中凋亡基因和CYP基因錶達變化,凋亡基因和CYP基因可能在TCE對肝細胞毒性效應中髮揮重要作用。
목적:연구삼록을희(TCE)대인간세포(L02세포)적조망기인(Caspase -3、Caspase-8、BAX、Bcl -2)화CYP기인(CYP1A2、CYP3A4、CYP2E1) mRNA표체수평적영향。방법:용함10%태우혈청적RPMI-1640배양간세포,DMSO작위용제대조,채용불동농도TCE(0.2、0.4、0.8、1.6、3.2 mmol/L)염독L02세포24 h ,령외용TCE 1.0 mmol/L염독세포불동시간(2h ,4h ,8h ,16h ,32h),채용Real-time형광정량PCR기술검측간세포중조망기인화CYP기인mRNA표체수평。결과:불동제량TCE염독후,조망기인Caspase-3、Caspase-8、BAX표체수평비대조조승고21%~145%,차이유통계학의의(P<0.05혹P<0.01),Bcl-2표체수평하강20%~35%。TCE염독인기CYP1A2、CYP3A4、CYP2E1적 mRNA표체명현상승,비대조조승고30%~550%,차이유통계학의의(P<0.05혹P<0.01)。용TCE 1.0 mmol/L염독2 h~32 h ,동양발현조망기인화CYP표체증가,여대조조비교차이유통계학의의(P<0.05,혹P<0.01)。결론:TCE가인기L02간세포중조망기인화CYP기인표체변화,조망기인화CYP기인가능재TCE대간세포독성효응중발휘중요작용。
Objective :To study mRNA expression of hepatic metabolic enzyme genes and apoptosis genes in L 02 cells after treatment with trichloroeth-ylene (TCE) .Methods :Liver cells (L02 cells) were cultured with various doses of TCE (0 .2 ,0 .4 ,0 .8 ,1 .6 ,and 3 .2 mmol/L) for 24h ,or the cells were cultured with a single dose of TCE (1 .0 mmol/L) for different periods of time (2h ,4h ,8h ,16h ,32h) ,the control was treated with DM-SO ,real-time fluorescent PCR assay was applied for detection of mRNA expression of apoptosis genes (Caspase-3、Caspase-8、BAX、Bcl-2) and hepatic metabolic enzyme genes (CYP1A2 ,CYP3A4 ,CYP2E1) .Results:The relative levels of mRNA expression of apoptosis genes and hepatic metabolic enzyme genes remarkably increased after TCE treatment at various doses (0 .2 ,0 .4 ,0 .8 ,1 .6 ,and 3 .2 mmol/L) for 24h ,the elevation of mRNA expression in TCE treatment groups was significant in comparison with control (P<0 .05 ,or P <0 .01) .Additionally ,when the cells were treated with a single dose of TCE (1 .0 mmol/L) for different periods of time (2h ,4h ,8h ,16h ,32h) ,the mRNA expression also increased signifi-cantly compared with control (P <0 .05 ,or P <0 .01) .Conclusions :Trichloroethylene could induce alternation of mRNA expression of apoptosis genes and hepatic metabolic enzyme genes ,these genes might play an important role in cytotoxicity of trichloroethylene .