安徽医药
安徽醫藥
안휘의약
ANHUI MEDICAL AND PHARMACEUTICAL JOURNAL
2014年
7期
1336-1339,1340
,共5页
刘永庆%童斯浩%鲍扬漪%王成宏
劉永慶%童斯浩%鮑颺漪%王成宏
류영경%동사호%포양의%왕성굉
原发性肝癌%塞来昔布%COX-2抑制剂%TRAIL%CIK
原髮性肝癌%塞來昔佈%COX-2抑製劑%TRAIL%CIK
원발성간암%새래석포%COX-2억제제%TRAIL%CIK
Hepatocellular Carcinoma%celecoxib%COX-2 inhibitor%TRAI-L,Cytokine-induced killer ( CIK)
目的：探讨选择性环氧合酶-2（COX-2）抑制剂塞来昔布（Celecoxib）对人肝癌HepG2和HepG3细胞株对肿瘤坏死因子相关的凋亡诱导配体（ TRAIL）的增敏作用及其可能机制。方法以人HepG2 HepG3肝癌细胞为研究对象，以塞来昔布和TRAIL作为干预手段，采用四甲基偶氮唑蓝（ MTT）法检测塞来昔布对肝癌细胞株的增殖抑制作用，采用流式细胞术，检测塞来昔布联合TRAIL对HepG2，HepG3细胞的凋亡及DR4，DR5的表达。塞来昔布与不同效靶比的细胞因子诱导的杀伤细胞（CIK）联合作用于人肝癌细胞株，经LDH检测法检测其杀伤作用。结果塞来昔布及TRAIL对人肝癌HepG2和HepG3细胞株均有显著抑制作用，塞来昔布联合TRAIL可显著增敏TRAIL对HepG2和HepG3的杀伤，其差异具有统计学意义（ P＜0．05），经过塞来昔布预处理的肝癌细胞更易被CIK细胞杀伤。结论塞来昔布对人肝癌HepG2和HepG3细胞株具有明显细胞毒性，联合TRAIL及CIK可显著增敏后者对肝癌细胞的杀伤效应，其机制可能与塞来昔布上调DR4，DR5有关。
目的：探討選擇性環氧閤酶-2（COX-2）抑製劑塞來昔佈（Celecoxib）對人肝癌HepG2和HepG3細胞株對腫瘤壞死因子相關的凋亡誘導配體（ TRAIL）的增敏作用及其可能機製。方法以人HepG2 HepG3肝癌細胞為研究對象，以塞來昔佈和TRAIL作為榦預手段，採用四甲基偶氮唑藍（ MTT）法檢測塞來昔佈對肝癌細胞株的增殖抑製作用，採用流式細胞術，檢測塞來昔佈聯閤TRAIL對HepG2，HepG3細胞的凋亡及DR4，DR5的錶達。塞來昔佈與不同效靶比的細胞因子誘導的殺傷細胞（CIK）聯閤作用于人肝癌細胞株，經LDH檢測法檢測其殺傷作用。結果塞來昔佈及TRAIL對人肝癌HepG2和HepG3細胞株均有顯著抑製作用，塞來昔佈聯閤TRAIL可顯著增敏TRAIL對HepG2和HepG3的殺傷，其差異具有統計學意義（ P＜0．05），經過塞來昔佈預處理的肝癌細胞更易被CIK細胞殺傷。結論塞來昔佈對人肝癌HepG2和HepG3細胞株具有明顯細胞毒性，聯閤TRAIL及CIK可顯著增敏後者對肝癌細胞的殺傷效應，其機製可能與塞來昔佈上調DR4，DR5有關。
목적：탐토선택성배양합매-2（COX-2）억제제새래석포（Celecoxib）대인간암HepG2화HepG3세포주대종류배사인자상관적조망유도배체（ TRAIL）적증민작용급기가능궤제。방법이인HepG2 HepG3간암세포위연구대상，이새래석포화TRAIL작위간예수단，채용사갑기우담서람（ MTT）법검측새래석포대간암세포주적증식억제작용，채용류식세포술，검측새래석포연합TRAIL대HepG2，HepG3세포적조망급DR4，DR5적표체。새래석포여불동효파비적세포인자유도적살상세포（CIK）연합작용우인간암세포주，경LDH검측법검측기살상작용。결과새래석포급TRAIL대인간암HepG2화HepG3세포주균유현저억제작용，새래석포연합TRAIL가현저증민TRAIL대HepG2화HepG3적살상，기차이구유통계학의의（ P＜0．05），경과새래석포예처리적간암세포경역피CIK세포살상。결론새래석포대인간암HepG2화HepG3세포주구유명현세포독성，연합TRAIL급CIK가현저증민후자대간암세포적살상효응，기궤제가능여새래석포상조DR4，DR5유관。
Objective To investigate the effect of COX-2 inhibitor celecoxib on the growth of hepatic carcinoma HepG 2 and HepG3 and explore its mechanism.Methods In human hepatic carcinoma cell line HepG2,HepG3 as the research object,using celecoxib and TRAIL as a means of intervention ,the function of celecoxib on cell proliferation inhibition was observed in different concentration and time by MTT,by using flow cytometric technique for the observation of celecoxib combined with TRAIL on hepatoma cell apoptosis and express change of DR4 and DR5.The apoptosis effect of Celecoxib combined with different concentrations CIK cells on hepatoma cells was detected by LDH assay .Results Celecoxib and TRAIL has obvious inhibition effect on hepatic carcinoma cell line HepG 2 and HepG3.Celecoxib would significantly sensitize TRAIL to HepG 2 and HepG3,it has statistical significance (P<0.05).Celecoxib pre-treatment made hepatoma cells more susceptible to CIK cell killing .Conclusion Celecoxib has obviously cytotoxic on hepatic carcino-ma cell line HepG2,HepG3.Celecoxib combine with TRAIL and CIK would significantly sensitize its cytotoxic effect .The mechanism may be associated with increased expression of DR 4/5 by celecoxib pretreatment .
