湖北农业科学
湖北農業科學
호북농업과학
2014年
9期
2181-2183,2184
,共4页
金钗石斛(Dendrobium nobide Lindl.)%快速繁殖%培养基%栽培基质
金釵石斛(Dendrobium nobide Lindl.)%快速繁殖%培養基%栽培基質
금차석곡(Dendrobium nobide Lindl.)%쾌속번식%배양기%재배기질
Dendrobium nobile Lindl.%rapid propagation%medium%transplantation medium
以野生金钗石斛(Dendrobium nobide Lindl.)茎段为试验材料,筛选启动培养时的最佳取材部位、最适基本培养基、生根培养基与栽培基质,研究金钗石斛快速繁殖的途径。结果表明,启动培养时用金钗石斛幼茎基部接种,在MS+6.0 mg/L 6-BA+0.2 mg/L NAA+100 mL/L椰乳+25 g/L蔗糖+6.3 g/L琼脂的培养基上效果最好,诱芽率可达93.00%。生根用MS+6.0 mg/L NAA+0.5 mg/L IBA+100 g/L香蕉+2.0 g/L活性炭+25 g/L蔗糖+6.3 g/L琼脂的培养基效果最好,生根率高达95%。最优的组培苗栽培基质是水苔+蛭石(3：2,V：V ),成活率高达90%。
以野生金釵石斛(Dendrobium nobide Lindl.)莖段為試驗材料,篩選啟動培養時的最佳取材部位、最適基本培養基、生根培養基與栽培基質,研究金釵石斛快速繁殖的途徑。結果錶明,啟動培養時用金釵石斛幼莖基部接種,在MS+6.0 mg/L 6-BA+0.2 mg/L NAA+100 mL/L椰乳+25 g/L蔗糖+6.3 g/L瓊脂的培養基上效果最好,誘芽率可達93.00%。生根用MS+6.0 mg/L NAA+0.5 mg/L IBA+100 g/L香蕉+2.0 g/L活性炭+25 g/L蔗糖+6.3 g/L瓊脂的培養基效果最好,生根率高達95%。最優的組培苗栽培基質是水苔+蛭石(3：2,V：V ),成活率高達90%。
이야생금차석곡(Dendrobium nobide Lindl.)경단위시험재료,사선계동배양시적최가취재부위、최괄기본배양기、생근배양기여재배기질,연구금차석곡쾌속번식적도경。결과표명,계동배양시용금차석곡유경기부접충,재MS+6.0 mg/L 6-BA+0.2 mg/L NAA+100 mL/L야유+25 g/L자당+6.3 g/L경지적배양기상효과최호,유아솔가체93.00%。생근용MS+6.0 mg/L NAA+0.5 mg/L IBA+100 g/L향초+2.0 g/L활성탄+25 g/L자당+6.3 g/L경지적배양기효과최호,생근솔고체95%。최우적조배묘재배기질시수태+질석(3：2,V：V ),성활솔고체90%。
The stems of wild Dendrobium nobile Lindl. were used as materials to select the optimal positions and the prefer-able medium for induction, root and transplantation. The results showed that the preferable production medium for inducing basal part of young stems was MS+6.0 mg/L 6-BA+0.2 mg/L NAA+100 mL/L coconut milk+25 g/L cane sugar +6.3 g/L agar, with buds induction rate of 93.00%. The preferable medium for rooting was MS+6.0 mg/L NAA+0.5 mg/L IBA+100 g/L ba-nana+2.0 g/L activated carbon+25 g/L cane sugar+6.3 g/L agar, with root induction rate of 95%. The optimal transplantation medium was sphagna+vermiculite(3:2,V:V ), with the survival rate of more than 90%.
