CAJ | 학술논문

利用高效液相色谱法测定贵定县本地茶种鸟王茶与引进的福鼎茶茶叶中的咖啡因和儿茶素含量,分析比较两种茶叶中咖啡因和儿茶素含量的差异,可为鸟王茶的保护与开发提供科学依据。采用C18色谱柱,流动相为NH4AC水溶液和甲醇,梯度洗脱,检测波长220 nm,流速为0.5 mL/min,柱温室温。结果表明,贵定鸟王茶与福鼎茶中的咖啡因含量无明显差异,鸟王茶儿茶素的单体化合物的含量明显高于福鼎茶。
이용고효액상색보법측정귀정현본지다충조왕다여인진적복정다다협중적가배인화인다소함량,분석비교량충다협중가배인화인다소함량적차이,가위조왕다적보호여개발제공과학의거。채용C18색보주,류동상위NH4AC수용액화갑순,제도세탈,검측파장220 nm,류속위0.5 mL/min,주온실온。결과표명,귀정조왕다여복정다중적가배인함량무명현차이,조왕다인다소적단체화합물적함량명현고우복정다。
The content of caffeine and catechins in Niaowang tea and Fuding tea were determinated by high performance liq-uid chromatography method. Niaowang tea was the local tea of Guiding country and Fuding tea was imported. The content of two ingredients in the two teas were analyzed and compared to provide the scientific basis for the protection and development of Niaowang tea. The separation of catechins and caffeine was achieved on a C18 analytical column. The mobile phase was consisted of NH4AC aqueous solution (A) and methanol (B). Gradient elution was performed with the wavelength at 200 nm, the flow rate of 0.5 mL/min and the column temperature of room temperature. The results showed that the content of caffeine in the two teas had little difference. The content of catechins of Niaowang tea was higher than that of Fuding tea.