湖北农业科学
湖北農業科學
호북농업과학
2014年
9期
2093-2096,2097
,共5页
张婉洁%何德%李翠新%李季
張婉潔%何德%李翠新%李季
장완길%하덕%리취신%리계
香菇(Lentinula edodes)%ISSR%遗传多样性%DNA聚类分析
香菇(Lentinula edodes)%ISSR%遺傳多樣性%DNA聚類分析
향고(Lentinula edodes)%ISSR%유전다양성%DNA취류분석
Lentinula edodes%ISSR%genetic diversity%DNA clustering analysis
采用ISSR分子标记技术对云南省大姚县、瑞丽市和四川省木里县的41株野生香菇(Lentinula edodes)菌株进行DNA聚类分析,利用NTSYS软件对其遗传多样性进行聚类分析。结果表明,利用18个引物对41个野生香菇菌株的DNA进行ISSR-PCR扩增,共扩增出162条清晰的DNA片段。聚类分析结果表明,相似水平在62%左右时,41株野生香菇菌株分为3个类群,各地香菇资源存在较丰富的遗传多样性且与地域无明显相关性。
採用ISSR分子標記技術對雲南省大姚縣、瑞麗市和四川省木裏縣的41株野生香菇(Lentinula edodes)菌株進行DNA聚類分析,利用NTSYS軟件對其遺傳多樣性進行聚類分析。結果錶明,利用18箇引物對41箇野生香菇菌株的DNA進行ISSR-PCR擴增,共擴增齣162條清晰的DNA片段。聚類分析結果錶明,相似水平在62%左右時,41株野生香菇菌株分為3箇類群,各地香菇資源存在較豐富的遺傳多樣性且與地域無明顯相關性。
채용ISSR분자표기기술대운남성대요현、서려시화사천성목리현적41주야생향고(Lentinula edodes)균주진행DNA취류분석,이용NTSYS연건대기유전다양성진행취류분석。결과표명,이용18개인물대41개야생향고균주적DNA진행ISSR-PCR확증,공확증출162조청석적DNA편단。취류분석결과표명,상사수평재62%좌우시,41주야생향고균주분위3개류군,각지향고자원존재교봉부적유전다양성차여지역무명현상관성。
The DNA fingerprinting of 41 wild strains of Lentinula edodes from three places Dayao country, Ruili country of Yunnan province and Muli country of Sichuan province were identified with ISSR-POR. The genetic diversity were analyzed by NTSYS-PC software. The results showed that 162 distinct DNA bands were produced from 18 pairs of primers. The results of clustering analysis showed that 41 strains were divided into 3 groups at similarity of 62%, implying that the wild strains of L.edodes had great genetic diversity and there was no obvious correlation with the geographical location.
