湖北农业科学
湖北農業科學
호북농업과학
2014年
9期
2070-2074
,共5页
‘红阳’猕猴桃%花青素%温度波动%A dA NS基因
‘紅暘’獼猴桃%花青素%溫度波動%A dA NS基因
‘홍양’미후도%화청소%온도파동%A dA NS기인
‘Hongyang’ kiwifruit%anthocyanin%temperature fluctuation%A dA NS
为明确‘红阳’猕猴桃果肉中花青素含量在季节性温度下的合成变化情况及花青素合成酶基因A dA NS表达的变化,以‘红阳’主产区四川蒲江和湖北武汉的‘红阳’猕猴桃为研究对象,测定其花青素的含量并克隆花青素合成基因A dA NS,并对其转录水平变化进行检测。结果表明,较高的季节性温度对果肉花青素的含量有抑制作用,在温度适宜的蒲江,A dA NS基因的表达水平与花青素积累变化相一致,而在高温的武汉其表达水平受到明显的抑制。
為明確‘紅暘’獼猴桃果肉中花青素含量在季節性溫度下的閤成變化情況及花青素閤成酶基因A dA NS錶達的變化,以‘紅暘’主產區四川蒲江和湖北武漢的‘紅暘’獼猴桃為研究對象,測定其花青素的含量併剋隆花青素閤成基因A dA NS,併對其轉錄水平變化進行檢測。結果錶明,較高的季節性溫度對果肉花青素的含量有抑製作用,在溫度適宜的蒲江,A dA NS基因的錶達水平與花青素積纍變化相一緻,而在高溫的武漢其錶達水平受到明顯的抑製。
위명학‘홍양’미후도과육중화청소함량재계절성온도하적합성변화정황급화청소합성매기인A dA NS표체적변화,이‘홍양’주산구사천포강화호북무한적‘홍양’미후도위연구대상,측정기화청소적함량병극륭화청소합성기인A dA NS,병대기전록수평변화진행검측。결과표명,교고적계절성온도대과육화청소적함량유억제작용,재온도괄의적포강,A dA NS기인적표체수평여화청소적루변화상일치,이재고온적무한기표체수평수도명현적억제。
To illustrate the changes of anthocyanin concentration and anthocyanin synthase under seasonal fluctuated temperature, the concentration of anthocyanin of ‘Hongyang’ kiwifruit from Pujiang, Sichuan and Wuhan, Hubei was measured under different seasonal temperatures. Anthocyanin synthase (A dA NS) was cloned. Quantitative real time PCR (RT-PCR) was used to detect the expression of A dA NS during fruit development. Result showed that seasonal high temperature had negative regulation on anthocyanin concentration in flesh. The expression level of A dA NS was positively cor related with anthocyanin synthesis in Pujiang with suitable temperature and was inhibited greatly in Wuhan with high temperature.
