CAJ | 학술논문

采用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法等分离纯化南海红树林内生真菌ZH-7的代谢产物,并通过理化常数测定和光谱分析鉴定其化学结构。结果表明,从南海红树林内生真菌ZH-7的培养液中分离得到6个代谢产物。经波谱解析,分别为：比卡菌素(1)、butyrolactone Ⅱ(2)、环(苯丙-脯)二肽(3)、环(苯丙-苯丙)二肽(4)、环(苯丙-缬)二肽(5)、环(苯丙-亮)二肽(6)。初步药理活性显示化合物1对口腔癌细胞KB,KBv200的抗肿瘤活性的IC50值分别为10.0和14.5μg/mL。通过鉴定,所有化合物均首次从南海红树林内生真菌ZH-7中分离得到。
채용반복규효주색보법、Sephadex LH-20응효색보법등분리순화남해홍수림내생진균ZH-7적대사산물,병통과이화상수측정화광보분석감정기화학결구。결과표명,종남해홍수림내생진균ZH-7적배양액중분리득도6개대사산물。경파보해석,분별위：비잡균소(1)、butyrolactone Ⅱ(2)、배(분병-포)이태(3)、배(분병-분병)이태(4)、배(분병-힐)이태(5)、배(분병-량)이태(6)。초보약리활성현시화합물1대구강암세포KB,KBv200적항종류활성적IC50치분별위10.0화14.5μg/mL。통과감정,소유화합물균수차종남해홍수림내생진균ZH-7중분리득도。
The compounds were isolated by column chromatography and identified on the basis of physic-chemical constants and spectral analysis to study the secondary metabolites of mangrove endophytic fungus No?ZH-7 from the South China Sea. The results showed that 6 metabolites were obtained and elucidated as Bikaverin (1), butyrolactone Ⅱ(2), cyclo-(Phe-pro) (3), cyclo-(Phe-phe) (4), cyclo-(Phe-Val) (5), cyclo-(Phe-leu) (6). In the preliminary bioassay, metabolite 1 showed strong inhibitory activity on the KB,KBv200 with IC50 values of 10.0 and 14.5 μg/mL, respectively. According to the Identification, all the metabolites were firstly obtained from endophytic fungus No?ZH-7.