CAJ | 학술논문

镜检观察菌株菌落及细胞形态,用正交试验设计法确定菌株最佳发酵条件。结果表明,该菌株与链霉菌形态类似,革兰氏染色呈阳性,最佳发酵条件为葡萄糖15 g/L,酵母液40 g/L,装液量30 mL/250 mL,发酵时间72 h。所产抗生素对高温(95℃)具有较高的稳定性,在酸性和中性环境中比较稳定。
경검관찰균주균락급세포형태,용정교시험설계법학정균주최가발효조건。결과표명,해균주여련매균형태유사,혁란씨염색정양성,최가발효조건위포도당15 g/L,효모액40 g/L,장액량30 mL/250 mL,발효시간72 h。소산항생소대고온(95℃)구유교고적은정성,재산성화중성배경중비교은정。
The colony and cell morphology of one strain was observed with microscopy. The orthogonal experiment was con-ducted to confirm the optimal fermentation conditions. The results showed that this strain was similar to Streptomyces on mor-phology. G+and the optimal fermentation conditions were glucose 15 g/L,yeast powder 40 g/L, loaded liquid 30 ml/250ml, fer-mentation time 72 h. The antibiotics produced by the actinomyces strain were highly stable in hot temperature (95℃), acidic or neutral environment.