CAJ | 학술논문

目的：探讨微小RNA（miR-126）在不同类型冠心病患者中的表达情况，为冠心病早期诊断、治疗及治疗后检测提供实验依据。方法将实验分成健康对照组（n=6）及冠心病组（n=36），对冠心病组（分为稳定性心绞痛组，不稳定性心绞痛组，非ST段抬高性心肌梗死组，ST段抬高性心肌梗死组，每组9例）接受经皮冠状动脉介入治疗（ PCI）术前，术后第1天，术后第5天等时间点的观察。应用实时荧光定量PCR（ qRT-qPCR）技术检测确诊为不同类型冠心病患者术前、
목적：탐토미소RNA（miR-126）재불동류형관심병환자중적표체정황，위관심병조기진단、치료급치료후검측제공실험의거。방법장실험분성건강대조조（n=6）급관심병조（n=36），대관심병조（분위은정성심교통조，불은정성심교통조，비ST단태고성심기경사조，ST단태고성심기경사조，매조9례）접수경피관상동맥개입치료（ PCI）술전，술후제1천，술후제5천등시간점적관찰。응용실시형광정량PCR（ qRT-qPCR）기술검측학진위불동류형관심병환자술전、
Objective ToinvestigatethemiR-126expressionindifferenttypesofpatientswithcoronaryheartdiseasedetection,topro-videexperimentalevidencefortheearlydiagnosisofcoronaryheartdisease,thetreatmentandaftertreatment.Methods Samplesweredividedin-to the control group(n=6)and the experimental group(patients with coronary heart disease,n=36). The experimental groups were treated with percutaneous coronary intervention treatment(PCI). These samples were observed preoperative and postoperative day 1,five days after same point in time. The expression of miR-126 of whole blood samples in the different patients( stable angina,unstable angina,ST-segment elevation my-ocardial infarction,non-ST-segment elevation myocardial infarction,each group 9 case)was detected by the application of real-time fluores-cence quantitative PCR( qRT-qPCR). Results ①The expression of miR-126 between the three groups( healthy control,stable angina,un-stable angina)was not significantly different,but it is significantly different and significantly reduced in the acute myocardial infarction group( in-cluding non-ST-segment elevation group,ST elevation group). ②There were significant differences( P <0. 05)of miR-126 levels at differ-ent times. The expression level of miR-126 in patients with ST-segment or non-ST-segment elevation myocardial infarction before operation was significantly lower than the that of after treatment. The expression gradually increased with the observation time. ③There is a significant inter-action about the expression level of miR-126 between time and different types of coronary heart disease( P <0. 05). The trend of the them was significantlydifferent.Conclusion ThemiR-126expressionwassignificantlydifferentinthedifferenttypesofcoronaryheartdiseaseandpa-tients with preoperative and postoperative. It may be provide experimental basis to the diagnosis of coronary heart disease and postoperative by the detection of miR-126.