CAJ | 학술논문

目的：探讨亚砷酸联合电离辐射对肝癌细胞株HepG2细胞周期及凋亡的影响。方法采用流式细胞仪观察5 mg/ml亚砷酸对肝癌细胞株HepG2细胞凋亡及细胞周期分布情况。结果肝癌细胞株HepG2细胞照射48 h后，对照组、照射组、亚砷酸组、亚砷酸+照射组细胞周期进行了重新分布，G0/G1期细胞比率降低，S期、G2/M期细胞比率增加；细胞凋亡率（%，x-± s ）分别为5.96±0.27、9.07±0.59、15.12±1.61、21.01±2.32，各组间有统计学差异（ F =63.41，P =0.000），两两比较均有统计学差异（ P <0.05）。结论亚砷酸对肝癌细胞株HepG2具有S期、G2/M期阻滞及诱导细胞凋亡的作用。
목적：탐토아신산연합전리복사대간암세포주HepG2세포주기급조망적영향。방법채용류식세포의관찰5 mg/ml아신산대간암세포주HepG2세포조망급세포주기분포정황。결과간암세포주HepG2세포조사48 h후，대조조、조사조、아신산조、아신산+조사조세포주기진행료중신분포，G0/G1기세포비솔강저，S기、G2/M기세포비솔증가；세포조망솔（%，x-± s ）분별위5.96±0.27、9.07±0.59、15.12±1.61、21.01±2.32，각조간유통계학차이（ F =63.41，P =0.000），량량비교균유통계학차이（ P <0.05）。결론아신산대간암세포주HepG2구유S기、G2/M기조체급유도세포조망적작용。
Objective Todiscusstheeffectsofarsenousacidincombinationwithionizingradiationonapoptosisandcycleofhepatocellular carcinomaHepG2cells.Methods Theeffectof5mg/mlarsenousacidinthehepatocellularcarcinomaHepG2cellswasobserved.Thecellapopto-sisandcellcycledistributionbyflowcytometerwereanalyzed.Results 48hafterirradiated,cellcycleredistributedinthecontrolgroup,theirradi-ation group,arsenous acid group and arsenic acid + irradiation groups. The G0/G1 phase cell proportion reduced,S phase and G2/M phases in-creased. The cell apoptosis rates(%,-x ± s )were 5. 96 ± 0. 27,9. 07 ± 0. 59,15. 12 ± 1. 61 and 21. 01 ± 2. 32,respectively. There were statistical differences between each groups( F =63. 41,P =0. 000),and have statistically significant between any two groups( P <0. 05). Conclusion ArsenousacidcanblockHepG2cellsofhepatocellularcarcinomaonSphaseandG2/Mphase.Aresnousacidalsocaninducecellapoptosis.