中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2014年
12期
766-770
,共5页
MGC-803细胞%雷替曲塞%5-Fu%p53%胃癌
MGC-803細胞%雷替麯塞%5-Fu%p53%胃癌
MGC-803세포%뢰체곡새%5-Fu%p53%위암
s:MGC-803%raltitrexed%5-fluorouracil%p53%gastric cancer
目的：初步探索雷替曲塞对人胃癌裸鼠皮下移植瘤生长的影响及其机制。方法：1）建立人胃癌细胞MGC-803裸鼠皮下移植瘤模型；2）药物干预后观察荷瘤鼠一般情况、体重、瘤重并计算抑瘤率；3）流式细胞仪测定瘤细胞的周期分布和凋亡；4）采用RT-PCR、Western blot印迹法检测各组肿瘤p53 mRNA及p53蛋白的表达水平。结果：经雷替曲塞及5-Fu作用后，与对照组相比，两组裸鼠的体重明显下降，瘤体积缩小，抑瘤率分别达到49.02%和45.75%。雷替曲塞组和5-Fu组瘤细胞的G0/G1期比例较对照组比例明显降低（P<0.01），而S期比例较对照组明显升高（P<0.01）。雷替曲塞组和5-Fu组裸鼠肿瘤p53 mRNA与蛋白表达水平均显著升高（P<0.01），但雷替曲塞组和5-Fu组之间并无差异。结论：1）雷替曲塞对人胃癌细胞MGC-803裸鼠移植瘤生长有抑制作用，且与5-Fu抑瘤效果相似；2）雷替曲塞可以诱导人胃癌细胞MGC-803裸鼠移植瘤细胞周期S期的阻滞，并诱导移植瘤细胞凋亡；3）雷替曲塞可通过上调p53 mRNA和蛋白的表达水平来发挥抑瘤作用。
目的：初步探索雷替麯塞對人胃癌裸鼠皮下移植瘤生長的影響及其機製。方法：1）建立人胃癌細胞MGC-803裸鼠皮下移植瘤模型；2）藥物榦預後觀察荷瘤鼠一般情況、體重、瘤重併計算抑瘤率；3）流式細胞儀測定瘤細胞的週期分佈和凋亡；4）採用RT-PCR、Western blot印跡法檢測各組腫瘤p53 mRNA及p53蛋白的錶達水平。結果：經雷替麯塞及5-Fu作用後，與對照組相比，兩組裸鼠的體重明顯下降，瘤體積縮小，抑瘤率分彆達到49.02%和45.75%。雷替麯塞組和5-Fu組瘤細胞的G0/G1期比例較對照組比例明顯降低（P<0.01），而S期比例較對照組明顯升高（P<0.01）。雷替麯塞組和5-Fu組裸鼠腫瘤p53 mRNA與蛋白錶達水平均顯著升高（P<0.01），但雷替麯塞組和5-Fu組之間併無差異。結論：1）雷替麯塞對人胃癌細胞MGC-803裸鼠移植瘤生長有抑製作用，且與5-Fu抑瘤效果相似；2）雷替麯塞可以誘導人胃癌細胞MGC-803裸鼠移植瘤細胞週期S期的阻滯，併誘導移植瘤細胞凋亡；3）雷替麯塞可通過上調p53 mRNA和蛋白的錶達水平來髮揮抑瘤作用。
목적：초보탐색뢰체곡새대인위암라서피하이식류생장적영향급기궤제。방법：1）건립인위암세포MGC-803라서피하이식류모형；2）약물간예후관찰하류서일반정황、체중、류중병계산억류솔；3）류식세포의측정류세포적주기분포화조망；4）채용RT-PCR、Western blot인적법검측각조종류p53 mRNA급p53단백적표체수평。결과：경뢰체곡새급5-Fu작용후，여대조조상비，량조라서적체중명현하강，류체적축소，억류솔분별체도49.02%화45.75%。뢰체곡새조화5-Fu조류세포적G0/G1기비례교대조조비례명현강저（P<0.01），이S기비례교대조조명현승고（P<0.01）。뢰체곡새조화5-Fu조라서종류p53 mRNA여단백표체수평균현저승고（P<0.01），단뢰체곡새조화5-Fu조지간병무차이。결론：1）뢰체곡새대인위암세포MGC-803라서이식류생장유억제작용，차여5-Fu억류효과상사；2）뢰체곡새가이유도인위암세포MGC-803라서이식류세포주기S기적조체，병유도이식류세포조망；3）뢰체곡새가통과상조p53 mRNA화단백적표체수평래발휘억류작용。
Objective:This study was designed to explore the effects and mechanisms of raltitrexed on the growth of the human gastric cancer cell line (MGC-803) transplanted in nude mice. Methods: Models of MGC-803 xenograft transplanted in nude mice were established. Body weight, mental state, food, and stool of the nude mice were closely monitored, and the inhibitory action in the tu-mors was evaluated after drug intervention. Distribution and apoptosis of the tumor cells were examined through flow cytometric assay. P53 mRNA and protein expression levels were determined through reverse transcription-polymerase chain reaction and Western blot analysis. Changes among the three groups were compared. Results:Body weights and tumor volumes of the nude mice were lower in the raltitrexed and 5-fluorouracil (5-Fu) groups than those in the control group. Tumor inhibition ratios were 49.02%and 45.75%in the raltitrexed and 5-Fu groups, respectively. In the G0/G1 stage, the number of cells decreased in the raltitrexed and 5-Fu groups. Signifi-cant differences were found between the experimental and control groups (P<0.01). In the S stage, the number of cells increased in the raltitrexed and 5-Fu groups, with significant differences from the control group (P<0.01). P53 mRNA and protein expression levels in the raltitrexed and 5-Fu groups were significantly higher than those in the control group (P<0.01), but no significant differences were found between the raltitrexed and 5-Fu groups (P>0.05). Conclusion:Raltitrexed can inhibit the growth of MGC-803 xenograft trans-planted in nude mice and shows inhibitory effect similar to that of 5-Fu. Raltitrexed can induce the S-phase block of the cell cycle and cell apoptosis in the MGC-803 xenografts in the nude mice. This drug may exhibit an antitumor effect by upregulating the p53 mRNA and protein expression levels.
