CAJ | 학술논문

目的：探讨鸦胆子油口服乳液对二乙基亚硝胺（DEN）诱导的大鼠原发性肝癌的抑制作用。方法：将大鼠随机分为正常对照组、模型对照组、鸦胆子油口服乳液组。采用自由饮水和灌胃给DEN的方法诱发大鼠肝癌模型，诱发肝癌第5周开始灌胃鸦胆子油口服液，5次/周，14周后处死大鼠，腹主动脉取血，全自动生化分析仪检测血清中ALT、AST、ALP、γ-GT和AFP的含量，观察大鼠肝系数、脾系数、胸腺系数、肝脏表面的癌结节数的变化，并进行影像学检查。结果：与正常对照组比较，模型对照组肝、脾指数和癌结节数明显增加，胸腺指数明显降低（P<0.01），血清中ALT、AFP、AST、ALP、γ-GT的水平显著高于正常对照组（P<0.01）；与模型组比较，鸦胆子油口服乳液显著降低肝癌大鼠肝指数、脾指数、癌结节数，升高胸腺指数（P<0.01），血清中ALT、AFP、AST、ALP、γ-GT的水平得到明显改善（P<0.01或P<0.05）。结论：鸦胆子油口服乳液对原发性肝癌具有显著抑制作用，并且作用机制为多方面。
목적：탐토아담자유구복유액대이을기아초알（DEN）유도적대서원발성간암적억제작용。방법：장대서수궤분위정상대조조、모형대조조、아담자유구복유액조。채용자유음수화관위급DEN적방법유발대서간암모형，유발간암제5주개시관위아담자유구복액，5차/주，14주후처사대서，복주동맥취혈，전자동생화분석의검측혈청중ALT、AST、ALP、γ-GT화AFP적함량，관찰대서간계수、비계수、흉선계수、간장표면적암결절수적변화，병진행영상학검사。결과：여정상대조조비교，모형대조조간、비지수화암결절수명현증가，흉선지수명현강저（P<0.01），혈청중ALT、AFP、AST、ALP、γ-GT적수평현저고우정상대조조（P<0.01）；여모형조비교，아담자유구복유액현저강저간암대서간지수、비지수、암결절수，승고흉선지수（P<0.01），혈청중ALT、AFP、AST、ALP、γ-GT적수평득도명현개선（P<0.01혹P<0.05）。결론：아담자유구복유액대원발성간암구유현저억제작용，병차작용궤제위다방면。
Objective: This study aimed to investigate the inhibitory effects of Brucea javanica oil oral emulsion (BJOOE) on primary liver cancer induced by diethylnitrosamine (DEN). Methods:Rats were randomly divided into the control group, model group, and BJOOE group. Rats were given free access to water. DEN was administered intragastrically to induce liver cancer in rats. Five weeks later, rats were intragastrically administered with BJOOE for five times per week. The rats were killed after 14 weeks. Abdominal aortic blood samples were collected. The contents of ALT, AST, ALP, γ-GT, and AFP of serum were detected by an automatic biochemical analyzer. The liver index, spleen index, thymus index, and changes in liver cancer nodules of the surface were observed in rats. Changes in the number of liver cancer nodules of the surface were detected by imaging. Results:Compared with the control group, the liver index, spleen index, and number of nodules of the model group significantly increased, whereas the thymus index significantly decreased (P<0.01). The levels of ALT, AFP, AST, ALP, andγ-GT of serum in the model group were significantly higher than those in the control group (P<0.01). Compared with the model group, BJOOE significantly reduced the liver index, spleen index, and number of cancer nodules, but increased the thymus index in the liver of rats with cancer (P<0.01). The levels of ALT, AFP, AST, ALP, andγ-GT of serum in rats with hepatic carcinoma significantly improved (P<0.01 or P<0.05). Conclusion:BJOOE could inhibit primary liver cancer, and the underlying mechanisms are complex.