CAJ | 학술논문

目的：建立川贝清肺糖浆的质量标准。方法采用薄层色谱（TLC）法对方中枇杷叶、甘草进行薄层色谱鉴别，对苦杏仁进行理化鉴别。采用反相高效液相色谱法测定甘草酸含量，色谱柱为 Discovery C18柱（150 mm ×4.6 mm，5μm），乙腈-水-冰醋酸（35：65：2）为流动相，流速为1 mL / min，柱温为27℃，检测波长为250 nm。结果薄层色谱中，供试品溶液在与对照药材溶液色谱相应位置上显相同颜色的斑点。甘草酸进样质量浓度0～224μg / mL 范围内与峰面积呈线性良好关系（ r ＝0.9999，n ＝6），平均加样回收率为98.92％， RSD ＝1.03％（ n ＝6）。结论该法简便、准确、可靠、专属性强、重现性好，可作为川贝清肺糖浆的质量标准。
목적：건립천패청폐당장적질량표준。방법채용박층색보（TLC）법대방중비파협、감초진행박층색보감별，대고행인진행이화감별。채용반상고효액상색보법측정감초산함량，색보주위 Discovery C18주（150 mm ×4.6 mm，5μm），을정-수-빙작산（35：65：2）위류동상，류속위1 mL / min，주온위27℃，검측파장위250 nm。결과박층색보중，공시품용액재여대조약재용액색보상응위치상현상동안색적반점。감초산진양질량농도0～224μg / mL 범위내여봉면적정선성량호관계（ r ＝0.9999，n ＝6），평균가양회수솔위98.92％， RSD ＝1.03％（ n ＝6）。결론해법간편、준학、가고、전속성강、중현성호，가작위천패청폐당장적질량표준。
Objective To establish the quality standard of Chuanbeiqingfei Syrup. Methods The TLC was used to conduct the identifi-cation of loquat leaves and glycyrrhiza,and the physiochemical identification of semen armeniacae amarae. The high performance liquid chromatography(HPLC)was adopted with the Discovery C18 column(150 mm × 4. 6 mm,5 μm);the mobile phase was acetonitrile -water - glacial acetic acid(35 : 65 : 2)with a flow rate of 1. 0 mL / min;the column temperature was 27 ℃ and the wavelength was set at 250 nm. Results In TLC,the test sample displayed the same color spots in the corresponding location of the reference sub-stance. The linear range of glycyrrhizic acid was 0 - 224 μg / mL( r = 0. 999 9,n = 6),and the average recovery rate was 98. 92%( RSD = 1. 03% ,n = 6). Conclusion The established method is simple,accurate and reliable with good specificity and good reproducibil-ity,and can be used as the quality standard of Chuanbeiqingfei Syrup.