CAJ | 학술논문

目的：探讨锌-α2-糖蛋白(ZAG)在成年雄性SD大鼠附睾管中的表达情况。方法：分别抽提附睾头、体、尾部精子和附睾液中的蛋白质，利用免疫印迹的方法研究ZAG在SD大鼠附睾管各段中的表达情况。结果：附睾头、体、尾部中的精子和附睾液中均有 ZAG 蛋白的存在，表达有一定的差异，附睾头部至尾部中的精子和附睾液表达逐渐增加。结论：ZAG主要分布在附睾尾部的精子和进一步的附睾液中，为后续的功能研究奠定了必要的基础。
목적：탐토자-α2-당단백(ZAG)재성년웅성SD대서부고관중적표체정황。방법：분별추제부고두、체、미부정자화부고액중적단백질，이용면역인적적방법연구ZAG재SD대서부고관각단중적표체정황。결과：부고두、체、미부중적정자화부고액중균유 ZAG 단백적존재，표체유일정적차이，부고두부지미부중적정자화부고액표체축점증가。결론：ZAG주요분포재부고미부적정자화진일보적부고액중，위후속적공능연구전정료필요적기출。
Objective:To explore the expression of Zn-alpha2-glycoprotein(ZAG)in epididymis tube from adult male SD rats. Methods:Sperm of epididymis head,body,tail and proteins of epididymal fluid were extracted respectively.We researched the expression of Zn-alpha2-glycoprotein(ZAG)in epididymis tube from SD rats using Western blot method.Results:There were ZAG protein in sperm of epididymis head,body,tail and epididymal fluid.There were some differences in expression.Expression is gradually increasing in sperm from the head to the tail of the epididymis and epididymal fluid.Conclusion:ZAG is mainly distributed in the sperm of tail of epididymis and epididymal fluid,and it set the foundation for further functional study.