CAJ | 학술논문

目的对比直接涂片法、细菌培养法、PCR检测法在淋病奈瑟氏菌检测中的应用效果。方法选取广东省惠州市惠东县慢性病防治站2011年9月～2013年9月收治的100例确诊或疑为淋病的患者作为研究对象，取100例患者的分泌物，分别使用直接涂片法、细菌培养法、PCR检测法进行检测，对比、分析3种检测方法的检测结果。结果 PCR检测的NG阳性率为81.0%，明显高于细菌培养法（61.0%）及直接涂片法（43.0%）（χ2=9.71、χ2=30.65），差异均具有统计学意义（P<0.05）；与直接涂片法相比，细菌培养法的NG阳性率更高（χ2=6.49），差异有统计学意义（P<0.05）。结论在直接涂片法、细菌培养法、PCR检测法3种检测法中，PCR检测法的灵敏度最高、特异性最强，是临床检测淋病的理想方法。
목적대비직접도편법、세균배양법、PCR검측법재임병내슬씨균검측중적응용효과。방법선취광동성혜주시혜동현만성병방치참2011년9월～2013년9월수치적100례학진혹의위임병적환자작위연구대상，취100례환자적분비물，분별사용직접도편법、세균배양법、PCR검측법진행검측，대비、분석3충검측방법적검측결과。결과 PCR검측적NG양성솔위81.0%，명현고우세균배양법（61.0%）급직접도편법（43.0%）（χ2=9.71、χ2=30.65），차이균구유통계학의의（P<0.05）；여직접도편법상비，세균배양법적NG양성솔경고（χ2=6.49），차이유통계학의의（P<0.05）。결론재직접도편법、세균배양법、PCR검측법3충검측법중，PCR검측법적령민도최고、특이성최강，시림상검측임병적이상방법。
Objective Contrast direct smear, bacterial culture, PCR assay application results in Neisseria gonorrhoeae detection.Methods Choose the hospital from September 2011 to September 2013 admitted 100 cases of gonorrhea diagnosed patients for the study, patients taking secretions 100 cases, respectively, using direct smear, bacterial culture, PCR assay for testing, comparing,analyzing test results of three detection methods.Results NG PCR testing positive rate was 81.0%, significantly higher than the bacterial culture (61.0%) and direct smear method (43.0%) (χ2= 9.71,χ2=30.65), the differences were statistically significant(P<0.05); compared with the direct smear method, NG-positive rate of bacterial culture and higher (χ2= 6.49), the difference was statistically significant(P<0.05).Conclusion In the direct smear, bacterial culture, PCR assay to detect three kinds of law, the maximum sensitivity of PCR assay specificity strongest, is the ideal way to clinical testing for gonorrhea.