CAJ | 학술논문

目的：研究罗格列酮（RGZ）逆转Burkitt淋巴瘤raji细胞对紫杉醇耐药的作用及机制。方法建立人Burkitt淋巴瘤raji紫杉醇耐药细胞株(raji/TAX 25)，细胞计数法检测raji/TAX 25对TAX的耐药指数和RGZ处理后各组raji细胞的增殖活性。流式细胞仪检测凋亡率，蛋白质印迹法（western blot）检测raji细胞耐药基因P-gp和多药耐药相关蛋白（MRP）的变化。结果成功建立了raji/TAX25耐药株、耐药指数为45.90，RGZ明显逆转Burkitt淋巴瘤raji细胞对紫杉醇的耐药性(P＜0.01)。western blot检测发现raji细胞P-gp和MRP表达显著下降(P＜0.05)。结论 RGZ具有逆转Burkitt淋巴瘤raji细胞对紫杉醇耐药的作用，其作用机制与下调P-gp和MRP表达有关。
목적：연구라격렬동（RGZ）역전Burkitt림파류raji세포대자삼순내약적작용급궤제。방법건립인Burkitt림파류raji자삼순내약세포주(raji/TAX 25)，세포계수법검측raji/TAX 25대TAX적내약지수화RGZ처리후각조raji세포적증식활성。류식세포의검측조망솔，단백질인적법（western blot）검측raji세포내약기인P-gp화다약내약상관단백（MRP）적변화。결과성공건립료raji/TAX25내약주、내약지수위45.90，RGZ명현역전Burkitt림파류raji세포대자삼순적내약성(P＜0.01)。western blot검측발현raji세포P-gp화MRP표체현저하강(P＜0.05)。결론 RGZ구유역전Burkitt림파류raji세포대자삼순내약적작용，기작용궤제여하조P-gp화MRP표체유관。
Objective To study the effects and mechanism of Rosiglitazone (RGZ) reversed Burkitt lymphoma Raji cells paclitaxel resistance. Methods Established human Burkitt lymphoma Raji paclitaxel resistant cell lines (raji/TAX25). Cell counting was used to observe the raji/TAX25 resistance index and the proliferation of raji cells deal with different concentrations of RGZ. Flow cytometry wasused to measure the apoptosis rate. western was used to measure the expression of cell resistance gene P-gp and MRP.Results Successfully established raji/TAX25 of drug-resistant strains, resistance index was 45.90, RGZ significantly reversed the Raji cells paclitaxel resistance (P<0.01). Decreased raji cells P-gp and MRP protein expression.Conclusion RGZ can reversed Burkitt lymphoma Raji cells paclitaxel resistance, its mechanism is related to inhibit P-gp and MRP protein protein expression.