CAJ | 학술논문

目的探究反应性胶质细胞增生、神经炎症与大鼠脑积水的关系.方法选取成年雄性SD大鼠35只,采用随机数字表法分为脑积水模型组(25只)、假手术组(5只)、正常假手术组(5只).采用高岭土脑室内注射法建立脑积水模型,术后3周进行磁共振检查,抽取脑脊液进行白细胞介素-18(IL-18)的酶联免疫吸附测定(ELISA)检测,并对脑组织的神经胶原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、同种异体移植炎症因子-1(allograft inflammatory factor-1,AIF-1)进行实时定量聚合酶链反应(RT-PCR)、蛋白免疫印迹(WB)和免疫组织化学检测,结果行统计学分析.结果造模组均有脑积水形成,与正常组和假手术组相比,造模组大鼠侧脑室体积明显增大(P＜0.05).GFAP、AIF-1、IL-18表达量在脑积水大鼠中均升高,且差异有统计学意义(P＜0.05).相关性分析显示GFAP、IL-18与脑积水严重程度呈正相关.结论反应性胶质细胞增生和神经炎症与脑积水严重程度呈正相关,有潜力成为脑积水发生发展的评价指标.能否通过抗感染和抑制胶质增生治疗脑积水,成为今后研究的新方向.
목적 탐구반응성효질세포증생、신경염증여대서뇌적수적관계.방법 선취성년웅성SD대서35지,채용수궤수자표법분위뇌적수모형조(25지)、가수술조(5지)、정상가수술조(5지).채용고령토뇌실내주사법건립뇌적수모형,술후3주진행자공진검사,추취뇌척액진행백세포개소-18(IL-18)적매련면역흡부측정(ELISA)검측,병대뇌조직적신경효원섬유산성단백(glial fibrillary acidic protein,GFAP)、동충이체이식염증인자-1(allograft inflammatory factor-1,AIF-1)진행실시정량취합매련반응(RT-PCR)、단백면역인적(WB)화면역조직화학검측,결과행통계학분석.결과 조모조균유뇌적수형성,여정상조화가수술조상비,조모조대서측뇌실체적명현증대(P＜0.05).GFAP、AIF-1、IL-18표체량재뇌적수대서중균승고,차차이유통계학의의(P＜0.05).상관성분석현시GFAP、IL-18여뇌적수엄중정도정정상관.결론 반응성효질세포증생화신경염증여뇌적수엄중정도정정상관,유잠력성위뇌적수발생발전적평개지표.능부통과항감염화억제효질증생치료뇌적수,성위금후연구적신방향.
Objective To investigate the relationship between reactive gliosis and hydrocephalus at both cellular and molecular levels.Methods Thirty-five SD rats were randomly divided into normal group (n =5),sham operation group (n =5) and model group (n =25).Hydrocephalic rat models were induced by intraventricular injections of 3％ kaolin and the ventricular dilatation were examined by MRI 3 weeks postoperatively.The model group were subdivided into the mild group (n =5),moderate group (n =7) and sever group (n =9) according to the degree of ventricular dilatation.Interleukin-18(IL-18),glial fibrillary acidic protein (GFAP),Iba-1 (ionized calcium-binding adaptor molecule)were detected by ELISA and RT-PCR.Immunohistochemistry,Western blot and correlation analysis were conducted.Results Compared to the normal group and the sham operation group,the ventricle of the model group was obviously enlarged(P＜0.05).The expression of GFAP and Iba-1 were increased(P＜0.05) in brain tissue in the model group and IL-18 was also increased in cerebral spinal fluid (CSF) sample.Correlation analysis revealed that the increase was positively correlated with the severity of ventricular dilatation.Conclusions These results indicate that gliosis and inflammation increase dramatically in experimental hydrocephalus and can be regarded as potential parameters for evaluating hydrocephalus.