水生生物学报
水生生物學報
수생생물학보
ACTA HYDROBIOLOGICA SINICA
2014年
4期
737-744
,共8页
DNA条形码%COⅠ基因%沙鳅亚科%物种鉴定
DNA條形碼%COⅠ基因%沙鰍亞科%物種鑒定
DNA조형마%COⅠ기인%사추아과%물충감정
DNA barcoding%COⅠ gene%Botiinae%Species identification
选择线粒体 COⅠ基因作为分子标记,进行沙鳅亚科鱼类(Botiinae)DNA 条形码及其分子系统发育研究。研究获得了沙鳅亚科7属19种共131个个体的COⅠ基因序列,利用MEGA5.0软件分析了沙鳅亚科鱼类COⅠ基因的序列特征,计算了种内及种间遗传距离。沙鳅亚科鱼类的分子系统发育关系的重建分别采用NJ法和Bayesian法。研究发现,沙鳅亚科COⅠ基因的碱基组成为：A 24.4%、T 29.5%、G 18.0%、C 28.1%。沙鳅亚科鱼类种内平均遗传距离为0.002±0.000,种间平均遗传距离为0.148±0.008。DNA条形码研究结果显示,所分析的19种沙鳅鱼类各自分别聚成单系分支,表明 COⅠ基因在本研究中具有100%的物种鉴别率。同时,系统发育分析支持各属的单系性,并且结果显示沙鳅亚科鱼类聚为两个分支,其中一支由薄鳅属和副沙鳅属构成,另一分支则包括：(沙鳅属、色鳅属)和[中华沙鳅属、(缨须鳅属、安彦鳅属)]。因此, COⅠ基因可以作为有效的分子标记对沙鳅亚科进行DNA条形码研究以及分子系统发育研究。
選擇線粒體 COⅠ基因作為分子標記,進行沙鰍亞科魚類(Botiinae)DNA 條形碼及其分子繫統髮育研究。研究穫得瞭沙鰍亞科7屬19種共131箇箇體的COⅠ基因序列,利用MEGA5.0軟件分析瞭沙鰍亞科魚類COⅠ基因的序列特徵,計算瞭種內及種間遺傳距離。沙鰍亞科魚類的分子繫統髮育關繫的重建分彆採用NJ法和Bayesian法。研究髮現,沙鰍亞科COⅠ基因的堿基組成為：A 24.4%、T 29.5%、G 18.0%、C 28.1%。沙鰍亞科魚類種內平均遺傳距離為0.002±0.000,種間平均遺傳距離為0.148±0.008。DNA條形碼研究結果顯示,所分析的19種沙鰍魚類各自分彆聚成單繫分支,錶明 COⅠ基因在本研究中具有100%的物種鑒彆率。同時,繫統髮育分析支持各屬的單繫性,併且結果顯示沙鰍亞科魚類聚為兩箇分支,其中一支由薄鰍屬和副沙鰍屬構成,另一分支則包括：(沙鰍屬、色鰍屬)和[中華沙鰍屬、(纓鬚鰍屬、安彥鰍屬)]。因此, COⅠ基因可以作為有效的分子標記對沙鰍亞科進行DNA條形碼研究以及分子繫統髮育研究。
선택선립체 COⅠ기인작위분자표기,진행사추아과어류(Botiinae)DNA 조형마급기분자계통발육연구。연구획득료사추아과7속19충공131개개체적COⅠ기인서렬,이용MEGA5.0연건분석료사추아과어류COⅠ기인적서렬특정,계산료충내급충간유전거리。사추아과어류적분자계통발육관계적중건분별채용NJ법화Bayesian법。연구발현,사추아과COⅠ기인적감기조성위：A 24.4%、T 29.5%、G 18.0%、C 28.1%。사추아과어류충내평균유전거리위0.002±0.000,충간평균유전거리위0.148±0.008。DNA조형마연구결과현시,소분석적19충사추어류각자분별취성단계분지,표명 COⅠ기인재본연구중구유100%적물충감별솔。동시,계통발육분석지지각속적단계성,병차결과현시사추아과어류취위량개분지,기중일지유박추속화부사추속구성,령일분지칙포괄：(사추속、색추속)화[중화사추속、(영수추속、안언추속)]。인차, COⅠ기인가이작위유효적분자표기대사추아과진행DNA조형마연구이급분자계통발육연구。
To analyze the characteristics of the sequences and to calculate the genetic distance within and between spe-cies, the 648 bp mitochondrial COⅠ gene was used as DNA barcode to analyze phylogenetic relationships of Botiinae fishes in 131 sequences from 19 species representing 7 genera of Botiinae. MEGA5.0 software was used in the current study. Phylogenetic analyses were carried out using neighbor-joining and Bayesian methods. Our result revealed that the nucleotide compositions were 24.4% A, 29.5% T, 18.0% G, and 28.1% C. The mean inter- and intra-specific genetic distances were 0.148±0.008 and 0.002±0.000, respectively. Individuals of each species were clustered into monophyletic groups based on the molecular phylogenetic tree, indicating that the mitochondria COⅠ gene is suitable for species discrimination. Moreover, the phylogenetic analyses supported monophyly of genera within Botiinae. Two clades were recovered within the subfamily Botiinae:one clade includes the sister-group of Leptobotia and Parabotia and the other contains Botia + Chromobotia and Sinibotia + (Syncrossus, Yasuhikotakia). This study indicated that the mitochondria COⅠ gene is a potentially effective marker for DNA barcoding and phylogenetic studies of Botiinae fishes.
