CAJ | 학술논문

以氧化豆油水溶物为试验材料,在草鱼肠道黏膜细胞培养液中加入不同浓度氧化豆油水溶物,研究不同剂量氧化豆油水溶物在不同作用时间下对肠道黏膜细胞生长、细胞形态结构的影响。结果显示：添加111.06-888.48 g/L氧化豆油的水溶物作用6h显著降低细胞活性(P<0.05),细胞集落面积减小、细胞形态改变,其中在444.24 g/L 氧化豆油的水溶物作用下培养细胞空泡变性,且脂肪滴沉积,线粒体肿胀。在222.12-888.48 g/L氧化豆油的水溶物作用下,3-9h内培养液中LDH活力显著增高(P<0.05)；各时间点培养液中GSH-PX、SOD、T-AOC均有显著变化。结果表明,在培养液中添加111.06-888.48 g/L氧化豆油的水溶物作用12h 内,对草鱼肠道黏膜细胞产生了损伤,表现为抑制细胞生长,改变细胞形态、结构,可能引起细胞膜脂质过氧化,导致膜结构破坏,且作用程度与添加浓度、作用时间相关。研究认为氧化豆油水溶物对草鱼肠道黏膜细胞具有显著性的损伤作用。
이양화두유수용물위시험재료,재초어장도점막세포배양액중가입불동농도양화두유수용물,연구불동제량양화두유수용물재불동작용시간하대장도점막세포생장、세포형태결구적영향。결과현시：첨가111.06-888.48 g/L양화두유적수용물작용6h현저강저세포활성(P<0.05),세포집락면적감소、세포형태개변,기중재444.24 g/L 양화두유적수용물작용하배양세포공포변성,차지방적침적,선립체종창。재222.12-888.48 g/L양화두유적수용물작용하,3-9h내배양액중LDH활력현저증고(P<0.05)；각시간점배양액중GSH-PX、SOD、T-AOC균유현저변화。결과표명,재배양액중첨가111.06-888.48 g/L양화두유적수용물작용12h 내,대초어장도점막세포산생료손상,표현위억제세포생장,개변세포형태、결구,가능인기세포막지질과양화,도치막결구파배,차작용정도여첨가농도、작용시간상관。연구인위양화두유수용물대초어장도점막세포구유현저성적손상작용。
The present study investigated the effects of water soluble matter from oxidized soybean oil on the growth, morphology and structure of intestinal epithelial cells (in vitro) from Ctenopharyngodon idella. The water soluble matter was applied at different concentrations for various periods of time. The activity and survival of cells were significant reduced after 6h incubation with culture medium supplemented with water soluble matter in the concentration range of 111.06-888.48 g/L. Furthermore there was an associated decrease in the size of cell colonies as well as a change in cell morphology. When the water soluble matter from oxidized soybean oil reached the concentration of 444.24 g/L, the formation of fat droplets and vesicles in cells could be observed under transmission electron microscope, as well as swelling mitochondria in cells. During 3-9h incubation, LDH activity significantly increased; there was also signi-ficant change in GSH-PX, SOD activity and T-AOC. In conclusion, during 12 hours the dissociated IECs from Cteno-pharyngodon idella could be damaged by the water soluble matter from oxidized soybean oil in the concentration range of 111.06-888.48 g/L. The damage occurs primarily in the manifestation of altered growth-inhibitory, morphology, and cell structure, probably involving the lipid peroxidation in cell membrane and the subsequent destruction in cell struc-ture. The extent of damage is correlated with the concentration and incubation time. Overall, there was significant damage of water soluble matter from oxidized soybean oil on IECs in vitro.