水生生物学报
水生生物學報
수생생물학보
ACTA HYDROBIOLOGICA SINICA
2014年
4期
681-688
,共8页
尹飞%龚颀杨%施兆鸿%孙鹏%彭士明%高权新%但学明%李安兴
尹飛%龔頎楊%施兆鴻%孫鵬%彭士明%高權新%但學明%李安興
윤비%공기양%시조홍%손붕%팽사명%고권신%단학명%리안흥
刺激隐核虫%褐菖%皮质醇%抗氧化酶%溶菌酶
刺激隱覈蟲%褐菖%皮質醇%抗氧化酶%溶菌酶
자격은핵충%갈창%피질순%항양화매%용균매
Cryptocaryon irritans%Sebastiscus marmoratus%Cortisol%Antioxidant enzyme%Lysozyme
为探明刺激隐核虫感染对褐菖生理机能的影响,研究分别用2500、5000、7500和10000幼虫/鱼的刺激隐核虫感染褐菖,并分别检测感染后24h、48h、72h 和96h各时间点血清中皮质醇(COR)、血糖(GLU)、总蛋白(TP)含量;肝脏中丙二醛(MDA)和维生素 C(VC)含量,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力;鳃和皮肤中溶菌酶(LZM)活力。结果显示,随着感染浓度的增加,血液中 COR 和 GLU 含量均出现不同程度的升高,其中2500、5000和7500幼虫/鱼组COR含量的最高值均出现在感染后第3天;而TP含量总体呈现逐步下降的趋势,尤其当感染浓度达到5000幼虫/鱼后, TP 含量的下降程度明显增加;肝脏中 MDA含量呈先降后升的变化趋势,其中24h、48h和72h 各点MDA含量的最大值均出现在10000幼虫/鱼组,最小值则集中出现于在2500和5000幼虫/鱼组;而VC含量则与MDA含量的趋势相反;SOD和CAT活力均出现不同程度的升高;鳃和皮肤 LZM 活力总体呈先上升后回落的变化趋势。综上可知,刺激隐核虫感染会对鱼体造成氧化胁迫和脂质过氧化反应,其严重程度与感染的虫细胞浓度相关。低浓度感染组的鱼所受胁迫较轻,在滋养体脱落后仍具有一定的自我修复能力;而高浓度感染组鱼免疫因子的释放受到抑制或出现紊乱,即便在虫体脱落后,其体质也很难恢复。
為探明刺激隱覈蟲感染對褐菖生理機能的影響,研究分彆用2500、5000、7500和10000幼蟲/魚的刺激隱覈蟲感染褐菖,併分彆檢測感染後24h、48h、72h 和96h各時間點血清中皮質醇(COR)、血糖(GLU)、總蛋白(TP)含量;肝髒中丙二醛(MDA)和維生素 C(VC)含量,超氧化物歧化酶(SOD)和過氧化氫酶(CAT)活力;鰓和皮膚中溶菌酶(LZM)活力。結果顯示,隨著感染濃度的增加,血液中 COR 和 GLU 含量均齣現不同程度的升高,其中2500、5000和7500幼蟲/魚組COR含量的最高值均齣現在感染後第3天;而TP含量總體呈現逐步下降的趨勢,尤其噹感染濃度達到5000幼蟲/魚後, TP 含量的下降程度明顯增加;肝髒中 MDA含量呈先降後升的變化趨勢,其中24h、48h和72h 各點MDA含量的最大值均齣現在10000幼蟲/魚組,最小值則集中齣現于在2500和5000幼蟲/魚組;而VC含量則與MDA含量的趨勢相反;SOD和CAT活力均齣現不同程度的升高;鰓和皮膚 LZM 活力總體呈先上升後迴落的變化趨勢。綜上可知,刺激隱覈蟲感染會對魚體造成氧化脅迫和脂質過氧化反應,其嚴重程度與感染的蟲細胞濃度相關。低濃度感染組的魚所受脅迫較輕,在滋養體脫落後仍具有一定的自我脩複能力;而高濃度感染組魚免疫因子的釋放受到抑製或齣現紊亂,即便在蟲體脫落後,其體質也很難恢複。
위탐명자격은핵충감염대갈창생리궤능적영향,연구분별용2500、5000、7500화10000유충/어적자격은핵충감염갈창,병분별검측감염후24h、48h、72h 화96h각시간점혈청중피질순(COR)、혈당(GLU)、총단백(TP)함량;간장중병이철(MDA)화유생소 C(VC)함량,초양화물기화매(SOD)화과양화경매(CAT)활력;새화피부중용균매(LZM)활력。결과현시,수착감염농도적증가,혈액중 COR 화 GLU 함량균출현불동정도적승고,기중2500、5000화7500유충/어조COR함량적최고치균출현재감염후제3천;이TP함량총체정현축보하강적추세,우기당감염농도체도5000유충/어후, TP 함량적하강정도명현증가;간장중 MDA함량정선강후승적변화추세,기중24h、48h화72h 각점MDA함량적최대치균출현재10000유충/어조,최소치칙집중출현우재2500화5000유충/어조;이VC함량칙여MDA함량적추세상반;SOD화CAT활력균출현불동정도적승고;새화피부 LZM 활력총체정선상승후회락적변화추세。종상가지,자격은핵충감염회대어체조성양화협박화지질과양화반응,기엄중정도여감염적충세포농도상관。저농도감염조적어소수협박교경,재자양체탈락후잉구유일정적자아수복능력;이고농도감염조어면역인자적석방수도억제혹출현문란,즉편재충체탈락후,기체질야흔난회복。
To investigate the effects of a Cryptocaryon irritans infection on the biochemical functions and immune re-sponses of the marbled rockfish Sebastiscus marmoratus, this study utilized C. at concentrations of 2,500, 5,000, 7,500, and 10,000 theronts/fish to infect marbled rockfish weighing (45±3) g. Within 96 hours after the infection we measured a variety of parameters at different time points, including (in the serum) cortisol (COR), glucose (GLU), total protein (TP) contens, (in the liver) the malondialdehyde (MDA) and vitamin C (VC) contents, the superoxide dismutase (SOD) and catalase (CAT) activities, and (in the gill and skin) the lysozyme (LZM) activities of the marbled rockfish. The re-sults showed that the serum COR and GLU contents of the marbled rockfish gradually increased along with the infection concentration and the lapse of time after the infection. However, the TP content decreased while infection concentration increased. Compared to the control, MDA contents in the liver decreased significantly in the 5 000 theronts/fish infec-tion group, then increased significantly and reached the maximum in the 10000 theronts/fish infection group. VC con-tents in the liver initially rose and then dropped. Compared to the control group, the SOD and CAT activities of the fish were significantly elevated. The LZM activities in the gill and skin initially increased and then decreased. In conclusion, C. irritans infection may result in adverse effects on the rockfish, probably through oxidative stress and lipid peroxida-tion. The physiological functions of the fish infected with low concentrations of C. irritans theronts could be effectively restored after the removal of trophont from the host; however the infection with high concentrations irreversibly im-paired the release of immune factors.