CAJ | 학술논문

为考察绿原酸对草鱼鱼种生长、非特异性免疫指标和肌肉品质的影响，在基础饲料中分别添加0.00%(对照组)、0.01%、0.02%、0.04%、0.06%、0.08%绿原酸，饲养平均体重(3.19±0.03) g的草鱼鱼种。经60d 饲养，各组草鱼增重率分别为283.1%、305.7%、307.3%、329.6%、308.3%、303.0%，饲料系数分别为1.45、1.33、1.33、1.26、1.30、1.36；与对照组相比，添加0.04%绿原酸显著提高草鱼鱼种增重率，降低了饲料系数(P<0.05)，添加0.01%、0.02%、0.06%绿原酸有提高草鱼鱼种增重率的趋势；在饲料中添加0.04%绿原酸可显著提高肝胰脏 SOD 活性(P<0.05)，添加0.08%绿原酸可显著降低血浆天门冬氨酸氨基转移酶活性(P<0.05)，各组肝胰脏溶菌酶、血浆碱性磷酸酶和丙氨酸氨基转移酶活性无显著差异(P>0.05)。对肌肉成分的分析表明，各组水分、粗脂肪含量无显著差异(P>0.05)，但添加0.04%绿原酸可显著提高肌肉粗蛋白、缬氨酸、异亮氨酸、组氨酸、必需氨基酸和总氨基酸含量(P<0.05)。添加0.02%绿原酸可显著提高肌肉羟脯氨酸、胶原蛋白含量(P<0.05)。综上所述，适量绿原酸能改善草鱼鱼种生长性能、非特异性免疫功能和肌肉品质。草鱼鱼种饲料中绿原酸添加量推荐为0.04%。
위고찰록원산대초어어충생장、비특이성면역지표화기육품질적영향，재기출사료중분별첨가0.00%(대조조)、0.01%、0.02%、0.04%、0.06%、0.08%록원산，사양평균체중(3.19±0.03) g적초어어충。경60d 사양，각조초어증중솔분별위283.1%、305.7%、307.3%、329.6%、308.3%、303.0%，사료계수분별위1.45、1.33、1.33、1.26、1.30、1.36；여대조조상비，첨가0.04%록원산현저제고초어어충증중솔，강저료사료계수(P<0.05)，첨가0.01%、0.02%、0.06%록원산유제고초어어충증중솔적추세；재사료중첨가0.04%록원산가현저제고간이장 SOD 활성(P<0.05)，첨가0.08%록원산가현저강저혈장천문동안산안기전이매활성(P<0.05)，각조간이장용균매、혈장감성린산매화병안산안기전이매활성무현저차이(P>0.05)。대기육성분적분석표명，각조수분、조지방함량무현저차이(P>0.05)，단첨가0.04%록원산가현저제고기육조단백、힐안산、이량안산、조안산、필수안기산화총안기산함량(P<0.05)。첨가0.02%록원산가현저제고기육간포안산、효원단백함량(P<0.05)。종상소술，괄량록원산능개선초어어충생장성능、비특이성면역공능화기육품질。초어어충사료중록원산첨가량추천위0.04%。
To investigate the effects of chlorogenic acid on the growth, non-specific immune function and the meat quality of juvenile grass carp, the diets were improved with the addition of chlorogenic acid at the concentrations of 0.00% (control group), 0.01%, 0.02%, 0.04%, 0.06%, and 0.08%. Fish with an average weight of (3.19±0.03) g were selected and fed for 60 days. The results showed that the growth rate of the six diet groups were 283.1%, 305.7%, 307.3%, 329.6%, 308.3%, 303.0%, and the FCR was 1.45, 1.33, 1.33, 1.26, 1.30, and 1.36, respectively. The growth rate and FCR were significantly improved with the addition of 0.04% chlorogenic acid compared with the control group (P<0.05). It was indicated that the growth yate was improved with the addition of chlorogenic acid at the concentrations of 0.01%, 0.02%, and 0.06%. SOD activities of hepato-pancreas increased significantly with 0.04% chlorogenic acid (P<0.05), and plasma AST activities decreased significantly with 0.08% chlorogenic acid (P<0.05). However, hepato-pancreas lysozyme, ALP and ALT of plasma activities were not affected by dietary chlorogenic acid (P>0.05). In terms of the muscle composition and the meat quality, there were no significant differences in muscle moisture and crude lipid contents among different diet groups. The muscle protein, Val, Ile, His, essential amino acids and total amino acids significantly increased (P<0.05) with the addition of 0.04% chlorogenic acid. Hydroxyproline and the collagen content of muscle increased (P<0.05) with the addition of 0.02% chlorogenic acid. In conclusion, the results above showed that the growth, non-specific immunity ability and the meat quality of juvenile grass carp could be improved with the addition of chlorogenic acid in diets. Therefore, the recommended level of chlorogenic acid in juvenile grass carp diet should be 0.04%.