CAJ | 학술논문

通过生长实验探求饲料中添加灵芝提取物饲料对异育银鲫(6.72±0.11) g生长、饲料利用及免疫应答的影响，以及停止灵芝提取物饲料后，使用效果的持续性。在实验进行的第21天(D21)、第46天(D46)、第65天(D65)进行取样，结果表明所有的灵芝提取物组的特定生长率在饲喂65d后与对照组无显著差异(P>0.05)。摄食率随着灵芝提取物在饲料中含量的增加而增加(P<0.05)，饲料效率则有着相反的趋势(P<0.05)。在 D46和 D65时，随着灵芝提取物的含量增高，吞噬活力略有增高，但无显著差异(P>0.05)。在 D21、D46和 D65时，呼吸暴发活力随着灵芝提取物的含量增高而增高(P<0.05)，在 D21时，(0.6%-21d、0%-44d)处理组和(3%-21d、0%-44d)处理组其呼吸暴发活力高于对照组(P<0.05)。在 D65时，(0.6%-21d、0%-44d)处理组和(3%-21d、0%-44d)处理组其呼吸暴发活力仍高于对照组(P>0.05)，但差异不显著。D21、D46和 D65时，替代途径补体溶血活力随着灵芝提取物的增高而降低(P<0.05)。所有处理组溶菌酶活力在D21时没有产生显著差异。在D46时，对照组溶菌酶活力比最高添加组低但高于低添加组(P<0.05)，(3%-1d、0%-44d)处理组也仍高于对照组(P<0.05)。在 D65时，所有添加组的溶菌酶活力均低于对照组(P<0.05)。在 D65时，髓过氧化物酶活力随着灵芝提取物的添加剂量升高而升高(P<0.05)，(0.6%-21d、0%-44d)处理组仍高于对照组(P<0.05)。灵芝提取物的能提高白细胞呼吸暴发活力，且在在高剂量摄入21d 后(D46时)，仍可以保持较高的活力，说明灵芝提取物对免疫应答有一定时间延续效应。随着灵芝提取物添加量的升高，异育银鲫在经爱德华氏菌攻毒后的存活率显著提高。最高存活率组为3%添加组，显著高于对照组(P<0.05)。实验周期为65d时，饲料中灵芝提取物推荐添加剂量为3%。
통과생장실험탐구사료중첨가령지제취물사료대이육은즉(6.72±0.11) g생장、사료이용급면역응답적영향，이급정지령지제취물사료후，사용효과적지속성。재실험진행적제21천(D21)、제46천(D46)、제65천(D65)진행취양，결과표명소유적령지제취물조적특정생장솔재사위65d후여대조조무현저차이(P>0.05)。섭식솔수착령지제취물재사료중함량적증가이증가(P<0.05)，사료효솔칙유착상반적추세(P<0.05)。재 D46화 D65시，수착령지제취물적함량증고，탄서활력략유증고，단무현저차이(P>0.05)。재 D21、D46화 D65시，호흡폭발활력수착령지제취물적함량증고이증고(P<0.05)，재 D21시，(0.6%-21d、0%-44d)처리조화(3%-21d、0%-44d)처리조기호흡폭발활력고우대조조(P<0.05)。재 D65시，(0.6%-21d、0%-44d)처리조화(3%-21d、0%-44d)처리조기호흡폭발활력잉고우대조조(P>0.05)，단차이불현저。D21、D46화 D65시，체대도경보체용혈활력수착령지제취물적증고이강저(P<0.05)。소유처리조용균매활력재D21시몰유산생현저차이。재D46시，대조조용균매활력비최고첨가조저단고우저첨가조(P<0.05)，(3%-1d、0%-44d)처리조야잉고우대조조(P<0.05)。재 D65시，소유첨가조적용균매활력균저우대조조(P<0.05)。재 D65시，수과양화물매활력수착령지제취물적첨가제량승고이승고(P<0.05)，(0.6%-21d、0%-44d)처리조잉고우대조조(P<0.05)。령지제취물적능제고백세포호흡폭발활력，차재재고제량섭입21d 후(D46시)，잉가이보지교고적활력，설명령지제취물대면역응답유일정시간연속효응。수착령지제취물첨가량적승고，이육은즉재경애덕화씨균공독후적존활솔현저제고。최고존활솔조위3%첨가조，현저고우대조조(P<0.05)。실험주기위65d시，사료중령지제취물추천첨가제량위3%。
To investigate the effects of the administration of a glucan extract from Ganoderma lucidum to gibel carp (Carassius auratus gibelio) on non-specific immune parameters, growth and disease resistance, 5 different treatments were conducted:a) a control diet without any glucan extract for 65 days (0%-65d);b) the control diet with 0.6%glucan extract for 21 days and then switched on the control diet for 44 days (0.6%-21d, 0%-44d);c) the control diet with 0.6%glucan extraction for 65 days (0.6%-65d);d) the control diet with 3.0%glucan extract for 21 days and then switched on the control diet for 44 days (3%-21d, 0%-44d); and e) the control diet with 3.0%glucan extract for 65 days (3%-65d). Each diet was allocated randomly to triplicate groups of fish with an average basal weight of 6.72 g, and each tank (300 L) was stocked with 45 fish. Growth, serum lysozyme, alternative pathway of complement hemolytic activity, blood leucocyte phagocytic activity, respiratory burst, and myeloperoxidase activity were monitored at Day 21, 46 and 65. We observed that glucan extract had no significant effects on s growth rate (SGR) at the end point of the feeding trials (P>0.05). In addition, dietary glucan extract increased the respiratory burst (P<0.05) while reduced the alternative pathway of complement hemolytic activity (P<0.05). The β-glucan extract enhanced leucocyte phagocyitc activity in a dose-dependent pattern (P>0.05). On Day 65, fish in Treatment C showed the highest survival rate for the challenge of Edwardsiella tarda (P<0.05). These results suggested that dietary 3.0% glucan extract supplementation may enhance immunity and disease resistance for gibel carp.