CAJ | 학술논문

预测并合成survivin-△Ex3 HLA-A2．1限制性CTL表位的基因疫苗，探讨携带表位基因的减毒鼠伤寒沙门氏菌口服疫苗对小鼠移植肝癌模型的保护作用。将表位基因序列连接，构建pVAX1-STEs-EGFP真核表达载体，转化入减毒鼠伤寒沙门氏菌作为口服疫苗。实验动物分为未免疫对照组、口服沙门氏菌组、pVAX1质粒转染的减毒鼠伤寒沙门氏菌组；口服pVAX1-Survivin-△3（T34A）！ EGFP质粒转染的的减毒鼠伤寒沙门氏菌组和口服pVAX1-STEs ！ EGFP质粒转染的的减毒鼠伤寒沙门氏菌组。结果表明：在5组肿瘤模型小鼠中，肿瘤瘤块平均直径分别为：15．11±2．43 mm、13．70±2．97 mm、13．05±1．77 mm、7．46±2．61 mm、9．05±2．18 mm；表位疫苗组与其他组相比，有显著性差异（P＜0．01）。说明小鼠口服携带survivin-△Ex3 HLA-A2．1限制性CTL表位的基因疫苗后，能抑制小鼠肝癌细胞的增殖和扩散。
예측병합성survivin-△Ex3 HLA-A2．1한제성CTL표위적기인역묘，탐토휴대표위기인적감독서상한사문씨균구복역묘대소서이식간암모형적보호작용。장표위기인서렬련접，구건pVAX1-STEs-EGFP진핵표체재체，전화입감독서상한사문씨균작위구복역묘。실험동물분위미면역대조조、구복사문씨균조、pVAX1질립전염적감독서상한사문씨균조；구복pVAX1-Survivin-△3（T34A）！ EGFP질립전염적적감독서상한사문씨균조화구복pVAX1-STEs ！ EGFP질립전염적적감독서상한사문씨균조。결과표명：재5조종류모형소서중，종류류괴평균직경분별위：15．11±2．43 mm、13．70±2．97 mm、13．05±1．77 mm、7．46±2．61 mm、9．05±2．18 mm；표위역묘조여기타조상비，유현저성차이（P＜0．01）。설명소서구복휴대survivin-△Ex3 HLA-A2．1한제성CTL표위적기인역묘후，능억제소서간암세포적증식화확산。
To predict and synthesize HLA-A2.1 restricted survivin-△Ex3-derived CTL epitope gene vaccine,and discuss the protective effects of oral attenuated salmonella vaccine with epitope gene on the model mice of transplanted hepatic cellular cancer. Epitope gene sequences with highest scores in prediction were connected to establish eukaryotic expression vector of pVAX1 -STEs-EGFP,which was then transferred into the attenuated salmonella to act as oral vaccine.Experimental animals were divided into the control group (un-vaccinated),oral salmonella group ,oral attenuated salmonella group (transfected by pVAX1 plasmid),oral atten-uated salmonella group (transfected by pVAX1 -Survivin -△3 (T34A)-EGFP plasmid)and oral attenuated salmonella group (transfected by pVAX1 -STEs-EGFP plasmid).The mice were given immunization and then injected liver cancer cells subcutane-ously.The size of the mass at the injection site was measured.In 5 groups of the mice,the average diameters of the tumor were respec-tively 15.11 ±2.43 mm,13.70 ±2.97 mm,13.05 ±1.77 mm,7.46 ±2.61 mm and 9.05 ±2.18 mm;Epitope gene vaccine groups have significant difference when compared with the other groups (P<0.01 ).We concluded that HLA-A2.1 restricted surviving-△Ex3-derived CTL epitope gene vaccine can inhibit the proliferation and migration of mice liver cancer cells after oral administration to mice.