体育学刊
體育學刊
체육학간
JOURNAL OF PHYSICAL EDUCATION
2014年
4期
140-144
,共5页
李方晖%肖琳%覃飞%刘承宜
李方暉%肖琳%覃飛%劉承宜
리방휘%초림%담비%류승의
运动生物化学%运动训练%骨骼肌%第三类去乙酰化酶%内稳态%老龄大鼠
運動生物化學%運動訓練%骨骼肌%第三類去乙酰化酶%內穩態%老齡大鼠
운동생물화학%운동훈련%골격기%제삼류거을선화매%내은태%노령대서
sports biochemistry%sports training%skeletal muscle%type 3 sirtuins%homeostasis%aged rat
观察8周中等强度低负荷量训练对老龄雌性大鼠腓肠肌Bax和Bcl-2蛋白水平及去乙酰化酶1(SIRT1)/去乙酰化酶3(SIRT3)轴基因信使核糖核酸(mRNA)表达的影响。16只18月龄雌性SD大鼠随机分为对照组和运动组(各8只)。运动组在跑台上以15 km/h(60%~75%VO2max)进行有氧运动,15 min/d,5 d/周,持续运动8周;对照组自由生活。第8周末运动后24 h宰杀并测定腓肠肌指数、蛋白免疫印迹法测定腓肠肌 Bax 和 Bcl-2蛋白水平;逆转录聚合酶链式反应(RT-PCR)测定SIRT3、SIRT1、锰超氧化物歧化酶(MnSOD)、半胱氨酸蛋白酶-3(Caspase-3)、过氧化物酶体增殖活化受体γ辅助活化因子-1α(PGC-1α)、线粒体转录因子 A(TFAM)和核呼吸因子1(NRF1) mRNA水平。结果显示,运动组腓肠肌质量(P<0.05)和腓肠肌指数均显著增加(P<0.01)、Bax蛋白水平显著降低(P<0.05),Bcl-2蛋白水平和Bcl-2/Bax值显著增加(P<0.05);运动组SIRT3、SIRT1、PGC-1α、NRF1、TFAM、MnSOD mRNA水平显著增加(P<0.05),Caspase-3 mRNA水平显著降低(P<0.05)。结果表明:中等强度低负荷训练可延缓老龄雌性大鼠肌细胞凋亡信号的改变;SIRT1/SIRT3轴介导的内稳态机制在中等强度低负荷训练提升老龄大鼠骨骼肌线粒体更新速率及抗氧化酶水平起重要作用。
觀察8週中等彊度低負荷量訓練對老齡雌性大鼠腓腸肌Bax和Bcl-2蛋白水平及去乙酰化酶1(SIRT1)/去乙酰化酶3(SIRT3)軸基因信使覈糖覈痠(mRNA)錶達的影響。16隻18月齡雌性SD大鼠隨機分為對照組和運動組(各8隻)。運動組在跑檯上以15 km/h(60%~75%VO2max)進行有氧運動,15 min/d,5 d/週,持續運動8週;對照組自由生活。第8週末運動後24 h宰殺併測定腓腸肌指數、蛋白免疫印跡法測定腓腸肌 Bax 和 Bcl-2蛋白水平;逆轉錄聚閤酶鏈式反應(RT-PCR)測定SIRT3、SIRT1、錳超氧化物歧化酶(MnSOD)、半胱氨痠蛋白酶-3(Caspase-3)、過氧化物酶體增殖活化受體γ輔助活化因子-1α(PGC-1α)、線粒體轉錄因子 A(TFAM)和覈呼吸因子1(NRF1) mRNA水平。結果顯示,運動組腓腸肌質量(P<0.05)和腓腸肌指數均顯著增加(P<0.01)、Bax蛋白水平顯著降低(P<0.05),Bcl-2蛋白水平和Bcl-2/Bax值顯著增加(P<0.05);運動組SIRT3、SIRT1、PGC-1α、NRF1、TFAM、MnSOD mRNA水平顯著增加(P<0.05),Caspase-3 mRNA水平顯著降低(P<0.05)。結果錶明:中等彊度低負荷訓練可延緩老齡雌性大鼠肌細胞凋亡信號的改變;SIRT1/SIRT3軸介導的內穩態機製在中等彊度低負荷訓練提升老齡大鼠骨骼肌線粒體更新速率及抗氧化酶水平起重要作用。
관찰8주중등강도저부하량훈련대노령자성대서비장기Bax화Bcl-2단백수평급거을선화매1(SIRT1)/거을선화매3(SIRT3)축기인신사핵당핵산(mRNA)표체적영향。16지18월령자성SD대서수궤분위대조조화운동조(각8지)。운동조재포태상이15 km/h(60%~75%VO2max)진행유양운동,15 min/d,5 d/주,지속운동8주;대조조자유생활。제8주말운동후24 h재살병측정비장기지수、단백면역인적법측정비장기 Bax 화 Bcl-2단백수평;역전록취합매련식반응(RT-PCR)측정SIRT3、SIRT1、맹초양화물기화매(MnSOD)、반광안산단백매-3(Caspase-3)、과양화물매체증식활화수체γ보조활화인자-1α(PGC-1α)、선립체전록인자 A(TFAM)화핵호흡인자1(NRF1) mRNA수평。결과현시,운동조비장기질량(P<0.05)화비장기지수균현저증가(P<0.01)、Bax단백수평현저강저(P<0.05),Bcl-2단백수평화Bcl-2/Bax치현저증가(P<0.05);운동조SIRT3、SIRT1、PGC-1α、NRF1、TFAM、MnSOD mRNA수평현저증가(P<0.05),Caspase-3 mRNA수평현저강저(P<0.05)。결과표명:중등강도저부하훈련가연완노령자성대서기세포조망신호적개변;SIRT1/SIRT3축개도적내은태궤제재중등강도저부하훈련제승노령대서골격기선립체경신속솔급항양화매수평기중요작용。
In order to observe the effects of 8-week medium intensity low load training on the levels of proteins Bax and Bcl-2 and the gene messenger RNA (mRNA) expression of axis sirtuin 1 (SIRT1)/sirtuin 3 (SIRT3) of gastrocne-mius of aged rats, the authors divided 16 18-month old female SD rats randomly into a control group and an exercise group, each of which contained 8 rats, let the rats in the exercise group do an aerobic exercise on a treadmill for con-secutive 8 weeks, at a speed of 15 km/h (with 60%~75%VO2max), 15 minutes a day, 5 days a week, let the rats in the control group live freely, in 24 hours after rat exercising at the end of week 8, killed the rats, measured gastrocnemius index, measured the levels of proteins Bax and Bcl-2 of gastrocnemius by means of Western blot analysis, measured the mRNA levels of SIRT3, SIRT1, manganese superoxide dismutase (MnSOD), Caspase 3, peroxisome prolifera-tor-activated receptor-γcoactivator-1 (PGC-1α), mitochondrial transcription factor A (TFAM) and nuclear respiratory factor 1 (NRF1) by means of RT-PCR, and revealed the following findings:as for the rats in the exercise group, their gastrocnemius mass and gastrocnemius index increased significantly (P<0.05 and P<0.01 respectively), their protein Bax level decreased significantly (P<0.05), their protein Bcl-2 level and Bcl-2/Bax ratio increased significantly (P<0.05);their mRNA levels of SIRT3, SIRT1, PGC-1α, NRF1, TFAM and MnSOD increased significantly (P<0.05), their mRNA level of Caspase-3 decreased significantly (P<0.05). The said findings indicated the followings:medium intensity low load training could delay the changing of muscle cell apoptosis signal of aged rats; the homeostatic mechanism mediated by axis SIRT1/SIRT3 played an important role in medium intensity low load training increasing the mitochondria refreshing rate and antioxidase level of skeletal muscle of aged rats.