CAJ | 학술논문

目的：通过观察SOX2、OCT4、β-连环蛋白和p120ctn蛋白在甲状腺滤泡型腺瘤和甲状腺腺癌组织中的表达、分布，分析4种蛋白与甲状腺癌的临床病理特征之间的关系，探讨其在甲状腺癌发生、发展和预后中的价值。方法收集10例正常甲状腺组织，20例甲状腺滤泡型腺瘤和78例甲状腺腺癌蜡块，应用免疫组织化学二步法，对其组织中的SOX2、OCT4、β-连环蛋白及p120ctn的表达情况进行检测，采用SPSS14.0软件进行统计学分析，分析4种蛋白的表达与患者的肿物大小、年龄、临床分期和淋巴结转移的关系。并分析SOX2、OCT4、β-连环蛋白和p120ctn的表达相关性。结果①20例甲状腺滤泡型腺瘤组织中，有3例SOX2表达阳性，有2例OCT4表达阳性，β-连环蛋白和p120ctn有2例和1例异常表达。②78例甲状腺癌组织中，SOX2和OCT4的阳性表达率是64%和87%，SOX2表达与淋巴结转移有明显相关关系（P<0.05）， OCT4的表达和淋巴结转移及肿瘤大小明显相关（P<0.05）。β-连环蛋白和p120ctn的异常表达率分别为79%和72%，二者的异常表达都和淋巴结转移相关（P<0.05）。③SOX2、OCT4、β-连环蛋白和p120ctn在甲状腺癌中的表达明显高于甲状腺滤泡腺瘤组织，其差异有统计学意义。（P<0.05）。④甲状腺癌的SOX2的异常表达强度随着OCT4表达强度的增强而升高，呈正相关（r=0.543）。β-连环蛋白异常表达与p120ctn异常表达呈正相关（r＝0.319）。结论①SOX2和OCT4蛋白在甲状腺癌中阳性表达率升高，β-连环蛋白和p120ctn在甲状腺癌中出现明显的表达异常，反映出其表达情况在甲状腺癌发生发展中起重要作用。②SOX2、OCT4、β-连环蛋白和p120ctn的表达和甲状腺癌病理特征关系紧密，这4种蛋白的表达情况可以用来监测甲状腺癌恶性程度。③SOX2与OCT4阳性表达，β-连环蛋白异常表达与p120ctn异常表达可能在甲状腺癌的发生发展中起协同作用，这4种蛋白可以对甲状腺癌的生物学行为进行准确预测，在制定个体化治疗方案中，也具有一定的临床价值。目的：通過觀察SOX2、OCT4、β-連環蛋白和p120ctn蛋白在甲狀腺濾泡型腺瘤和甲狀腺腺癌組織中的錶達、分佈，分析4種蛋白與甲狀腺癌的臨床病理特徵之間的關繫，探討其在甲狀腺癌髮生、髮展和預後中的價值。方法收集10例正常甲狀腺組織，20例甲狀腺濾泡型腺瘤和78例甲狀腺腺癌蠟塊，應用免疫組織化學二步法，對其組織中的SOX2、OCT4、β-連環蛋白及p120ctn的錶達情況進行檢測，採用SPSS14.0軟件進行統計學分析，分析4種蛋白的錶達與患者的腫物大小、年齡、臨床分期和淋巴結轉移的關繫。併分析SOX2、OCT4、β-連環蛋白和p120ctn的錶達相關性。結果①20例甲狀腺濾泡型腺瘤組織中，有3例SOX2錶達暘性，有2例OCT4錶達暘性，β-連環蛋白和p120ctn有2例和1例異常錶達。②78例甲狀腺癌組織中，SOX2和OCT4的暘性錶達率是64%和87%，SOX2錶達與淋巴結轉移有明顯相關關繫（P<0.05）， OCT4的錶達和淋巴結轉移及腫瘤大小明顯相關（P<0.05）。β-連環蛋白和p120ctn的異常錶達率分彆為79%和72%，二者的異常錶達都和淋巴結轉移相關（P<0.05）。③SOX2、OCT4、β-連環蛋白和p120ctn在甲狀腺癌中的錶達明顯高于甲狀腺濾泡腺瘤組織，其差異有統計學意義。（P<0.05）。④甲狀腺癌的SOX2的異常錶達彊度隨著OCT4錶達彊度的增彊而升高，呈正相關（r=0.543）。β-連環蛋白異常錶達與p120ctn異常錶達呈正相關（r＝0.319）。結論①SOX2和OCT4蛋白在甲狀腺癌中暘性錶達率升高，β-連環蛋白和p120ctn在甲狀腺癌中齣現明顯的錶達異常，反映齣其錶達情況在甲狀腺癌髮生髮展中起重要作用。②SOX2、OCT4、β-連環蛋白和p120ctn的錶達和甲狀腺癌病理特徵關繫緊密，這4種蛋白的錶達情況可以用來鑑測甲狀腺癌噁性程度。③SOX2與OCT4暘性錶達，β-連環蛋白異常錶達與p120ctn異常錶達可能在甲狀腺癌的髮生髮展中起協同作用，這4種蛋白可以對甲狀腺癌的生物學行為進行準確預測，在製定箇體化治療方案中，也具有一定的臨床價值。
목적：통과관찰SOX2、OCT4、β-련배단백화p120ctn단백재갑상선려포형선류화갑상선선암조직중적표체、분포，분석4충단백여갑상선암적림상병리특정지간적관계，탐토기재갑상선암발생、발전화예후중적개치。방법수집10례정상갑상선조직，20례갑상선려포형선류화78례갑상선선암사괴，응용면역조직화학이보법，대기조직중적SOX2、OCT4、β-련배단백급p120ctn적표체정황진행검측，채용SPSS14.0연건진행통계학분석，분석4충단백적표체여환자적종물대소、년령、림상분기화림파결전이적관계。병분석SOX2、OCT4、β-련배단백화p120ctn적표체상관성。결과①20례갑상선려포형선류조직중，유3례SOX2표체양성，유2례OCT4표체양성，β-련배단백화p120ctn유2례화1례이상표체。②78례갑상선암조직중，SOX2화OCT4적양성표체솔시64%화87%，SOX2표체여림파결전이유명현상관관계（P<0.05）， OCT4적표체화림파결전이급종류대소명현상관（P<0.05）。β-련배단백화p120ctn적이상표체솔분별위79%화72%，이자적이상표체도화림파결전이상관（P<0.05）。③SOX2、OCT4、β-련배단백화p120ctn재갑상선암중적표체명현고우갑상선려포선류조직，기차이유통계학의의。（P<0.05）。④갑상선암적SOX2적이상표체강도수착OCT4표체강도적증강이승고，정정상관（r=0.543）。β-련배단백이상표체여p120ctn이상표체정정상관（r＝0.319）。결론①SOX2화OCT4단백재갑상선암중양성표체솔승고，β-련배단백화p120ctn재갑상선암중출현명현적표체이상，반영출기표체정황재갑상선암발생발전중기중요작용。②SOX2、OCT4、β-련배단백화p120ctn적표체화갑상선암병리특정관계긴밀，저4충단백적표체정황가이용래감측갑상선암악성정도。③SOX2여OCT4양성표체，β-련배단백이상표체여p120ctn이상표체가능재갑상선암적발생발전중기협동작용，저4충단백가이대갑상선암적생물학행위진행준학예측，재제정개체화치료방안중，야구유일정적림상개치。
Objective To study the distribution and expression of SOX2, OCT4, β-catenin and p120ctn in thy-roid adenoma and thyroid carcinoma, to explore the relationship between the clinical and pathological features of these markers, and to analyze their significance in the pathogenesis, development and prognosis of thyroid carcinoma. Methods We recruited 20 patients with thyroid adenoma, 78 with thyroid carcinoma and 10 healthy subjects. The two-step immunohistochemistry was applied to determine the expression of SOX2, OCT4, β-catenin and p120ctn. SPSS 14.0 software package was employed to conduct statistical analyses. This entailed the assessment of the correlation be-tween expression of these markers and tumor size, age, lymph node metastasis status and tumor staging. Results Of the 20 thyroid adenoma tissues, positive expression of SOX2 was found in three cases, OCT4 in 2 cases, β-catenin in 2 cases and p120ctn in a single case. The positivity of OCT4 and SOX2 was 87%and 64%, respectively. The expres-sion of SOX2 was significantly associated with lymph node metastasis and pathological grading (both P<0.05). The ex-pression of OCT4 was significantly related to the tumor size and lymph node metastasis status (both P<0.05). The ab-normal expression ratio of p120ctn and β-catenin was 72% and 79%, respectively. The abnormal expression of these two markers were significantly related to the lymph node metastasis (both P<0.05). In thyroid carcinoma, the expres-sion levels of SOX2, OCT4, β-catenin and p120ctn were much higher than those in thyroid adenoma (all P<0.05). There was a trend of increased abnormal expression of OCT4 with the increasing intensity of SOX2 expression ( r=0.543). The abnormal expression of β-catenin was significantly correlated with the p120ctn expression (r=0.319). Con-clusions In thyroid carcinoma, the expression of OCT4 and SOX2 is increased and both p120ctn and β-catenin demonstrate aberrant expression, suggesting that these markers might have played an important role in the pathogene-sis. The expression of these markers is significantly correlated with the pathological charateristics of thyroid carcino-ma, indicating that they may help monitor the degree of malignancy of thyroid carcinoma. The expression of p120ctn and β-catenin and the aberrant expression of OCT4 and SOX2 may have a synergistic influence on the growth of thy-roid carcinoma. These markers offer the crucial tool for predicting the biological behavior of thyroid carcinoma and confer clinical significance for establishing the individualized therapeutic protocols.