CAJ | 학술논문

目的：探讨内分泌治疗后前列腺癌患者肿瘤组织中上皮间质转化（EMT）标志物表达的变化。方法收集山西医科大学第一医院2006年1月至2012年12月收治的17例经过内分泌治疗前后的前列腺癌患者肿瘤组织标本，采用免疫组织化学链霉亲和素-生物素复合物（SABC）法检测上皮钙黏素（E Cadherin），波形蛋白（Vimentin）、神经钙黏素（N Cadherin）等分子标志物的表达。结果经过内分泌治疗后，前列腺癌患者肿瘤组织中上皮细胞标志E Cadherin表达阳性率由（87．8±5．4）％下降到（6．3±1．7）％，间充质细胞标志N Cadherin表达阳性率由（1．2±0．6）％增加到（46．1±5．6）％，Vimentin表达阳性率由（0．61±0．24）％增加到（28．53±3．52）％，经配对样本t检验，按a＝0．05水准，差异均具有统计学意义（P均＜0．001）。结论前列腺癌内分泌治疗控制肿瘤生长一段时间后，可以导致EMT特点的细胞数量增加，肿瘤侵袭转移能力增强，这可能是现有内分泌治疗最终失败的原因之一。
목적：탐토내분비치료후전렬선암환자종류조직중상피간질전화（EMT）표지물표체적변화。방법수집산서의과대학제일의원2006년1월지2012년12월수치적17례경과내분비치료전후적전렬선암환자종류조직표본，채용면역조직화학련매친화소-생물소복합물（SABC）법검측상피개점소（E Cadherin），파형단백（Vimentin）、신경개점소（N Cadherin）등분자표지물적표체。결과경과내분비치료후，전렬선암환자종류조직중상피세포표지E Cadherin표체양성솔유（87．8±5．4）％하강도（6．3±1．7）％，간충질세포표지N Cadherin표체양성솔유（1．2±0．6）％증가도（46．1±5．6）％，Vimentin표체양성솔유（0．61±0．24）％증가도（28．53±3．52）％，경배대양본t검험，안a＝0．05수준，차이균구유통계학의의（P균＜0．001）。결론전렬선암내분비치료공제종류생장일단시간후，가이도치EMT특점적세포수량증가，종류침습전이능력증강，저가능시현유내분비치료최종실패적원인지일。
Objective Imploring the change of the biomarkers of epithelial- mesenchymal transition (EMT) in human prostate cancer tissue after endocrine therapy. Methods 17 pairs of human prostate cancer tissue before and after endocrine therapy were collected in First Hospital of Shanxi Medical University from Jan 2006 to Dec 2012. Im-munohistochemistry SABC was used to detect the expression of EMT markers E Cadherin, N Cadherin and Vimentin in the cancer cells. Results After endocrine therapy, the number of E Cadherin positive cells decreased from (87.8± 5.4)% to (6.3±1.7)%.The number of N Cadherin positive cells increased from (1.2±0.6)% to (46.1±5.6)%. The number of Vimentin positive cells increased from (0.61±0.24)% to (28.53±3.52)%. The difference had significance (P<0.001). Conclusion Endocrine therapy can control the growth of the tumor for a period, meanwhile the number the EMT positive cells increases, which promotes the invasion and metastasis of the tumor. This phenomenon could be one of the reasons of failure of existing endocrine therapy.