CAJ | 학술논문

目的：利用DBA/2小鼠移植舌癌荷瘤模型，探究OK-432肿瘤细胞疫苗对舌癌的抑制作用。方法以戊二醛为交联剂，使KLN-205细胞与OK-432产生交联，制备OK-432肿瘤细胞疫苗；将DBA/2小鼠随机分为4组，每组20只，分别右侧腹部皮下注射0.9%氯化钠注射液、OK-432、灭活的KLN-205细胞、OK-432疫苗，接种KLN-205肿瘤细胞后观察对比舌癌瘤体大小，并用统计学分析其结果。结果肿瘤大小除KLN-205组与对照组间差异没有统计学意义（P＝0．094）外，其余各组间差异均有统计学意义，其中OK-432疫苗组肿瘤体积均小于其他3组。结论 OK-432肿瘤细胞疫苗能抑制小鼠舌癌的生长，具有一定的抗肿瘤效应。
목적：이용DBA/2소서이식설암하류모형，탐구OK-432종류세포역묘대설암적억제작용。방법이무이철위교련제，사KLN-205세포여OK-432산생교련，제비OK-432종류세포역묘；장DBA/2소서수궤분위4조，매조20지，분별우측복부피하주사0.9%록화납주사액、OK-432、멸활적KLN-205세포、OK-432역묘，접충KLN-205종류세포후관찰대비설암류체대소，병용통계학분석기결과。결과종류대소제KLN-205조여대조조간차이몰유통계학의의（P＝0．094）외，기여각조간차이균유통계학의의，기중OK-432역묘조종류체적균소우기타3조。결론 OK-432종류세포역묘능억제소서설암적생장，구유일정적항종류효응。
Objective To investigate the inhibitory effects of OK-432 tumor cell vaccine on tongue cancer growth in DBA/2 mice by using the tongue cancer xenograft models. Methods The OK-432 tumor cell vaccine was prepared by inducing conjugation of OK-432 to squamous cell carcinoma (KLN-205) with 0.2% glutaraldehyde (GA). The DBA/2 mice were randomly allocated to receive subcutaneous injection of saline ( n=20), OK-432 (n=20), inacti-vated KLN-205 cells (n=20) and OK-432 tumor cell vaccine (n=20) on the right abdomen. This allowed assessment of the tumor size before and after KLN-205 cell inoculation. Results The tumor size was significantly different among the four groups except for OK-432 vaccine group compared with control group (P=0.094). Inoculation with OK-432 vaccine resulted in marked reduction in the tumor size compared with the remaining three groups. Conclusion OK-432 tumor cell vaccine can inhibit the growth of tongue cancer in mice, suggesting the potential anti-tumor effects.