CAJ | 학술논문

目的：观察活血生肌外用中药对大鼠失神经支配创面角蛋白19（k19）阳性表达的影响。方法168只SD大鼠随机分成龙血膏组、生肌膏组、失神经组和正常组，每组42只。对前三组大鼠制造背部失神经支配的深创面模型，之后各组创面分别以龙血生肌膏、生肌愈皮膏、凡士林外用治疗；正常组大鼠背部制造深创面模型，以凡士林治疗。观察不同时间点各组创面愈合率、创面组织k19阳性表达率。结果各时间点正常组两中药组创面愈合率均高于失神经组（P<0.05，P<0.01）。龙血膏组、生肌膏组k19阳性表达于第11天达峰值，失神经组和正常组第15天达峰值。第4、7、11、15天时龙血膏组、生肌膏组k19阳性表达率明显高于失神经组和正常组（P<0.05，P<0.01），失神经组低于正常组（P<0.05，P<0.01）。结论活血生肌外用中药对失神经支配创面的愈合有促进作用，可能与外用中药增强了失神经支配创面的表皮干细胞增殖与分化有关。
목적：관찰활혈생기외용중약대대서실신경지배창면각단백19（k19）양성표체적영향。방법168지SD대서수궤분성룡혈고조、생기고조、실신경조화정상조，매조42지。대전삼조대서제조배부실신경지배적심창면모형，지후각조창면분별이룡혈생기고、생기유피고、범사림외용치료；정상조대서배부제조심창면모형，이범사림치료。관찰불동시간점각조창면유합솔、창면조직k19양성표체솔。결과각시간점정상조량중약조창면유합솔균고우실신경조（P<0.05，P<0.01）。룡혈고조、생기고조k19양성표체우제11천체봉치，실신경조화정상조제15천체봉치。제4、7、11、15천시룡혈고조、생기고조k19양성표체솔명현고우실신경조화정상조（P<0.05，P<0.01），실신경조저우정상조（P<0.05，P<0.01）。결론활혈생기외용중약대실신경지배창면적유합유촉진작용，가능여외용중약증강료실신경지배창면적표피간세포증식여분화유관。
Objective To investigate the effect of the external use of Chinese medicine on the expression of Keratin 19(K19) in denervated wound healing of rats. Methods One hundred and sixty-eight SD rats were ran-domly divided into 4 groups: A group(longxuegao group),B group(shengjigao group),C group(denervated group), and D group(normal group),42 rats in each group. A wound with denervated whole layer skin defection was estab-lished on the back of every rat in A,B,and C groups,while a wound with whole layer skin defection was estab-lished on the back of every rat in D group. The rat in A and B groups were externally treated with the corre-sponding drugs,and the rat in C and D groups were treated with vaseline. The healing rates of wound were calcu-lated and the level of K19 expression was determined by immunohistochemistry at different time points after inter-vention. Results The healing rate of A,B,and D groups were higher than that of C group(P<0.05). The level of K19 expression of A and B groups reached the peak on d11,which was earlier than that of C and D groups(on d15).On d4,d7,d11,d15,the level of K19 expression of A and B groups was significantly higher than that of C and D groups(P<0.05 or P<0.01),and that of C group was lower than that of D group(P<0.05 or P<0.01). Con-clusion The external use of Chinese medicine can promote wound healing of denervation. Its mechanism may be related to enhancing proliferation and differentiation of epidermal stem cells in the healing process of the wound of denervation.