CAJ | 학술논문

建立了高效液相色谱法准确、高效测定油脂混合体系中β-谷甾醇含量的方法，并对油脂混合物中β-谷甾醇的提取工艺进行了优化。采用C18反相色谱柱对β-谷甾醇含量进行测定，流动相为甲醇-水(体积比98．5：1．5)，检测波长210 nm。结果表明：β-谷甾醇在25~500μg/mL范围内，质量浓度与峰面积呈良好的线性关系(R2=0．9998)，仪器检出限为7．5μg/mL，相对标准偏差(RSD)为0．85%；油脂混合物中β-谷甾醇的最佳提取工艺条件为提取次数4次，离心转速2000 r/min，皂化温度80℃，皂化时间210 min；在最佳提取工艺条件下β-谷甾醇的回收率为93．44%~96．48%，RSD为1．70%。
건립료고효액상색보법준학、고효측정유지혼합체계중β-곡치순함량적방법，병대유지혼합물중β-곡치순적제취공예진행료우화。채용C18반상색보주대β-곡치순함량진행측정，류동상위갑순-수(체적비98．5：1．5)，검측파장210 nm。결과표명：β-곡치순재25~500μg/mL범위내，질량농도여봉면적정량호적선성관계(R2=0．9998)，의기검출한위7．5μg/mL，상대표준편차(RSD)위0．85%；유지혼합물중β-곡치순적최가제취공예조건위제취차수4차，리심전속2000 r/min，조화온도80℃，조화시간210 min；재최가제취공예조건하β-곡치순적회수솔위93．44%~96．48%，RSD위1．70%。
The accurate and efficient method for determing β-sitosterol content of oil mixture by HPLC was established,and the extraction process of β-sitosterol from oil mixture was optimized. C18 reverse-phase chromatography column was used for the determination ofβ-sitosterol content under the conditions of mobile phase methanol - water (volume ratio 98. 5:1. 5 ) and detection wavelength 210 nm. The re-sults showed that the mass concentration of β-sitosterol had a good linear relationship with peak area in the range of 25-500μg/mL. The correlation coefficient, the instrumental limit of detection and the rela-tive standard deviation (RSD) were 0. 999 8, 7. 5 μg/mL and 0. 85% respectively. The optimal extrac-tion conditions of β-sitosterol from oil mixture were obtained as follows:extraction times four, centrifugal speed 2 000 r/min, saponification temperature 80℃ and saponification time 210 min. Under these conditions,the recovery rate and RSD of β-sitosterol were 93. 44% -96. 48% and 1. 70%, respectively.