天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2014年
7期
680-682
,共3页
杨景红%董祖海%肖福斌%候巧燕
楊景紅%董祖海%肖福斌%候巧燕
양경홍%동조해%초복빈%후교연
钙黏着糖蛋白类%胆管肿瘤%免疫组织化学%印迹法,蛋白质%神经型钙黏蛋白%肝门部胆管癌
鈣黏著糖蛋白類%膽管腫瘤%免疫組織化學%印跡法,蛋白質%神經型鈣黏蛋白%肝門部膽管癌
개점착당단백류%담관종류%면역조직화학%인적법,단백질%신경형개점단백%간문부담관암
cadherins%bile duct neoplasms%immunohistochemistry%blotting,Western%neural cadherin%perihilar cholangiocarcinoma
目的：研究神经型钙黏蛋白（N-cadherin）在肝门部胆管癌组织中的表达及临床意义。方法免疫组化检测26例肝门部胆管癌与癌旁及5例正常胆管组织中N-cadherin表达差异。Western blot对比N-cadherin在肝门部胆管癌组织中与癌旁中差异。结果免疫组化示26例肝门部胆管癌癌组织中有14例N-cadherin表达阳性，癌旁组织中6例阳性（P<0.05）。正常胆管阳性表达0例。N-cadherin在低分化癌组阳性表达率高于中、高分化癌组。Western blot示N-cadherin在肝门部胆管癌组织中的表达高于癌旁组织(0.88±0.12 vs 0.43±0.09,P<0.05)。结论 N-cadherin可能参与肝门部胆管癌的发生发展。
目的：研究神經型鈣黏蛋白（N-cadherin）在肝門部膽管癌組織中的錶達及臨床意義。方法免疫組化檢測26例肝門部膽管癌與癌徬及5例正常膽管組織中N-cadherin錶達差異。Western blot對比N-cadherin在肝門部膽管癌組織中與癌徬中差異。結果免疫組化示26例肝門部膽管癌癌組織中有14例N-cadherin錶達暘性，癌徬組織中6例暘性（P<0.05）。正常膽管暘性錶達0例。N-cadherin在低分化癌組暘性錶達率高于中、高分化癌組。Western blot示N-cadherin在肝門部膽管癌組織中的錶達高于癌徬組織(0.88±0.12 vs 0.43±0.09,P<0.05)。結論 N-cadherin可能參與肝門部膽管癌的髮生髮展。
목적：연구신경형개점단백（N-cadherin）재간문부담관암조직중적표체급림상의의。방법면역조화검측26례간문부담관암여암방급5례정상담관조직중N-cadherin표체차이。Western blot대비N-cadherin재간문부담관암조직중여암방중차이。결과면역조화시26례간문부담관암암조직중유14례N-cadherin표체양성，암방조직중6례양성（P<0.05）。정상담관양성표체0례。N-cadherin재저분화암조양성표체솔고우중、고분화암조。Western blot시N-cadherin재간문부담관암조직중적표체고우암방조직(0.88±0.12 vs 0.43±0.09,P<0.05)。결론 N-cadherin가능삼여간문부담관암적발생발전。
Objective To explore the expression and the clinical significance of neural cadherin (N-cadherin) in perihilar cholangiocarcinoma. Methods The expression of N-cadherin was detected by immunohistochemical method in 26 patients with perihilar cholangiocarcinoma and 5 samples of normal bile duct tissues. The expression of N-cadherin was also compared by Western blotting assay between perihilar cholangiocarcinoma and paraneoplastic tissues. Results The im-munohistochemical results showed that positive expression of N-cadherin was observed in 14 of 26 patients with perihilar cholangiocarcinoma and in 6 of 26 paraneoplastic samples (53.84%vs 23.08%, P<0.05). There was 0 N-cadherin expres-sion in normal bile duct tissues. The positive rate of N-cadherin expression was higher in low differentiated group than that in middle and well differentiated groups. The result of Western blotting assay showed that N-cadherin expression was signifi-cantly higher in perihilar cholangiocarcinoma than that in the paraneoplastic tissues (0.88 ± 0.12 vs 0.43 ± 0.09, P < 0.05). Conclusion N-cadherin may be involved in the occurrence and development of perihilar cholangiocarcinoma.
