CAJ | 학술논문

研究生物酶催化废白土油与甲醇酯交换制备生物柴油的最佳工艺条件。通过对比相当用量的Lipozyme TL IM和Novozyme 435的催化效果，筛选出Lipozyme TL IM为适宜的酶；在此基础上，以醇油摩尔比、生物酶添加量、反应温度、反应时间为自变量，生物柴油得率为响应值，进行酯交换制备生物柴油的响应面优化实验。结果表明，酯交换反应最佳条件为：醇油摩尔比4：1， Lipozyme TL IM添加量10%(以废白土油质量计)，反应温度35℃，反应时间15 h；在此条件下，生物柴油得率为95．9%，所得生物柴油非常接近0#柴油的质量标准。
연구생물매최화폐백토유여갑순지교환제비생물시유적최가공예조건。통과대비상당용량적Lipozyme TL IM화Novozyme 435적최화효과，사선출Lipozyme TL IM위괄의적매；재차기출상，이순유마이비、생물매첨가량、반응온도、반응시간위자변량，생물시유득솔위향응치，진행지교환제비생물시유적향응면우화실험。결과표명，지교환반응최가조건위：순유마이비4：1， Lipozyme TL IM첨가량10%(이폐백토유질량계)，반응온도35℃，반응시간15 h；재차조건하，생물시유득솔위95．9%，소득생물시유비상접근0#시유적질량표준。
The optimal production conditions of biodiesel by transesterification of waste clay oil and metha-nol catalyzed by enzyme were studied. The catalysis effects of equal dosage of two enzymes( Lipozyme TL IM and Novozyme 435)were compared,and Lipozyme TL IM was screened as the suitable catalyst. With molar ratio of methanol to oil, enzyme dosage, reaction temperature and reaction time as independent var-iables, biodiesel yield as response value, the production of biodiesel from waste clay oil catalyzed by en-zyme was optimized by response surface methodology. The results showed that the optimal transesterifica-tion conditions were obtained as follows:molar ratio of methanol to oil 4:1 , Lipozyme TL IM dosage 10%( based on the mass of waste clay oil ) , reaction temperature 35℃and reaction time 15 h;under these conditions, the biodiesel yield was 95. 9%,and the quality of the biodiesel was very close to the quality standard of 0 # diesel.