CAJ | 학술논문

采用单因素试验研究不同温度、光照强度和氮质量浓度对大龙骨藻生长与油脂合成的影响。结果表明：大龙骨藻生长适宜的温度为20~30℃，最适温度为25℃；低温有利于EPA和DHA含量提高，10℃时EPA和DHA含量分别占总脂肪酸的(27．98±0．97)%和(3．02±0．64)%；生长适宜的光照强度为32~48μmol/(m2·s)，而总脂含量在光照强度为80μmol/(m2·s)时最高，达(34．66±0．86)%；低光照强度利于多不饱和脂肪酸( PUFA)含量提高，高光照强度利于EPA含量提高；不同氮质量浓度对生长和总脂含量影响显著(P<0．05)，生长适宜的氮质量浓度为20~30 mg/L，最适氮质量浓度为25 mg/L，缺氮则能促进总脂含量提高；高氮质量浓度利于PUFA和EPA的合成，随着氮质量浓度的增加，EPA和PUFA含量明显增加，氮质量浓度为25 mg/L时，EPA和PUFA含量最高，分别达(24．67±0．44)%和(40．55±0．58)%。
채용단인소시험연구불동온도、광조강도화담질량농도대대용골조생장여유지합성적영향。결과표명：대용골조생장괄의적온도위20~30℃，최괄온도위25℃；저온유리우EPA화DHA함량제고，10℃시EPA화DHA함량분별점총지방산적(27．98±0．97)%화(3．02±0．64)%；생장괄의적광조강도위32~48μmol/(m2·s)，이총지함량재광조강도위80μmol/(m2·s)시최고，체(34．66±0．86)%；저광조강도리우다불포화지방산( PUFA)함량제고，고광조강도리우EPA함량제고；불동담질량농도대생장화총지함량영향현저(P<0．05)，생장괄의적담질량농도위20~30 mg/L，최괄담질량농도위25 mg/L，결담칙능촉진총지함량제고；고담질량농도리우PUFA화EPA적합성，수착담질량농도적증가，EPA화PUFA함량명현증가，담질량농도위25 mg/L시，EPA화PUFA함량최고，분별체(24．67±0．44)%화(40．55±0．58)%。
The effects of different temperatures, light intensities and mass concentrations of nitrogen on the growth and lipid synthesis of Tropidoneis maxima were studied. The results showed that the suitable temper-ature for the growth of Tropidoneis maxima was 20-30℃ and the optimal temperature was 25℃; lower temperature could promote the accumulation of EPA and DHA;when the temperature was 10℃, the con-tents of DHA and EPA achieved (3. 02 ± 0. 64)% and (27. 98 ± 0. 97)% respectively;the proper light in-tensity for the growth of Tropidoneis maxima was 32-48 μmol/( m2 ·s) , however, the total lipid content was the highest when the light intensity was 80 μmol/(m2·s), reaching (34. 66 ± 0. 86)%; lower light intensity was benefit for the algae to synthesize polyunsaturated fatty acids(PUFA), while higher light in-tensity helped the algae to increase EPA content;different mass concentrations of nitrogen had great effects on the growth and total lipid content (P<0. 05), the appropriate mass concentration of nitrogen for the growth of Tropidoneis maxima was 20-30 mg/L and 25 mg/L was the best, but nitrogen deficiency could increase the total lipid content;higher mass concentration of nitrogen promoted the synthesis of EPA and PUFA, and the contents of EPA and PUFA increased significantly with the increase of the mass concentra-tion of nitrogen;when the mass concentration of nitrogen was 25 mg/L, the contents of EPA and PUFA were the highest, reaching (24. 67 ± 0. 44)% and (40. 55 ± 0. 58)% respectively.