CAJ | 학술논문

研究了不同的油脂提取工艺对产油酵母油脂产率的影响。以高产油酵母JM-D为原料，以油脂产率为测定指标，比较了蜗牛酶法、超声波破碎法、反复冻融法、细胞匀浆机破碎法、微波破碎法、研磨法以及酸热法对酵母细胞壁的破碎效果，并对有机溶剂提取法与索氏抽提法提取细胞内油脂的效果进行比较。结果显示，蜗牛酶法的细胞破碎效果最好，油脂产率高达46．2%；其次是反复冻融(100℃水浴解冻)法，油脂产率高达43．4%；研磨法+酸热法的油脂提取效果也较好，油脂产率达到37．8%。研磨后，采用索氏抽提法的提取效果优于有机溶剂提取法的。
연구료불동적유지제취공예대산유효모유지산솔적영향。이고산유효모JM-D위원료，이유지산솔위측정지표，비교료와우매법、초성파파쇄법、반복동융법、세포균장궤파쇄법、미파파쇄법、연마법이급산열법대효모세포벽적파쇄효과，병대유궤용제제취법여색씨추제법제취세포내유지적효과진행비교。결과현시，와우매법적세포파쇄효과최호，유지산솔고체46．2%；기차시반복동융(100℃수욕해동)법，유지산솔고체43．4%；연마법+산열법적유지제취효과야교호，유지산솔체도37．8%。연마후，채용색씨추제법적제취효과우우유궤용제제취법적。
The effects of different oil extraction processes on oil productivity of oil-producing yeast were studied. With high oil-producing yeast strains JM-D as raw material and oil productivity as indicator, the crushing effects of JM-D cell wall by different methods ( snail enzymatic method,ultrasonic crushing method,repeated freeze-thaw method, cell homogenizer crushing method, microwave crushing method, grinding method and acid-hot method) were compared,and the extraction effects of oil from JM-D cell by organic solvent extraction method and Soxhlet extraction method were also compared. The results showed that the crushing effect of JM-D cell wall by snail enzymatic method was the best,the oil produc-tivity reaching 46. 2%,followed by repeated freeze-thaw method(thawing in 100℃water bath) and acid-hot method combined with grinding method,and their oil productivities reached 43. 4% and 37. 8% respec-tively . The extraction effect of oil from JM-D cell after grinding by Soxhlet extraction method was superior to that by organic solvent extraction method.