CAJ | 학술논문

目的：检测溃疡性结肠炎（UC）患者血浆中髓源性抑制细胞（MDSCs）水平，并探讨其临床意义。方法选取50例UC患者为研究组，选择同期50例健康体检者为对照组，应用流式细胞仪检测两组外周血MDSCs和CD4＋CD25＋调节性T细胞（CD4＋CD25＋Treg）水平，并分析UC患者外周血MDSCs与CD4＋CD25＋Treg、白细胞（WBC）计数和血小板（PLT）计数的相关性。结果与对照组比较，研究组患者外周血CD4＋CD25＋Treg明显降低（P＜0．05），MDSCs比例、WBC计数和PLT计数明显升高（P＜0．05）。与UC缓解期比较，UC活动期患者外周血CD4＋CD25＋Treg比例明显降低（P＜0．05），MDSCs 比例、WBC计数和PLT计数明显升高（P＜0．05）。 UC患者MDSCs与WBC计数、PLT计数呈正相关（P＜0．05），与CD4＋CD25＋Treg之间呈负相关（P＜0．05）。结论 UC患者外周血MDSCs水平明显升高，与UC患者病情活动度有关，检测MDSCs有助于UC发病机制的认识和辅助诊断。
목적：검측궤양성결장염（UC）환자혈장중수원성억제세포（MDSCs）수평，병탐토기림상의의。방법선취50례UC환자위연구조，선택동기50례건강체검자위대조조，응용류식세포의검측량조외주혈MDSCs화CD4＋CD25＋조절성T세포（CD4＋CD25＋Treg）수평，병분석UC환자외주혈MDSCs여CD4＋CD25＋Treg、백세포（WBC）계수화혈소판（PLT）계수적상관성。결과여대조조비교，연구조환자외주혈CD4＋CD25＋Treg명현강저（P＜0．05），MDSCs비례、WBC계수화PLT계수명현승고（P＜0．05）。여UC완해기비교，UC활동기환자외주혈CD4＋CD25＋Treg비례명현강저（P＜0．05），MDSCs 비례、WBC계수화PLT계수명현승고（P＜0．05）。 UC환자MDSCs여WBC계수、PLT계수정정상관（P＜0．05），여CD4＋CD25＋Treg지간정부상관（P＜0．05）。결론 UC환자외주혈MDSCs수평명현승고，여UC환자병정활동도유관，검측MDSCs유조우UC발병궤제적인식화보조진단。
Objective To detect the level of plasma myeloid-derived suppressor cells ( MDSCs ) in patients with ulcerative colitis ( UC ) , and to investigate its clinical significance .Methods Fifty cases of UC were chosen as research group,and 50 healthy cases who accepted physical examination were chosen as control group .The correlation of MDSCs in peripheral blood of UC patients with CD 4 +CD25 +Treg,WBC count and PLT count was analyzed .Results Comparing with control group , the CD4 +CD25 +Treg in the peripheral blood of patients in the research group decreased significantly ( P<0 .05 ) , and the proportion of MDSCs , WBC count and PLT count increased significantly ( P<0 .05 ) .The proportion of CD4+CD25+Treg in peripheral blood of patients with active UC decreased was significantly lower than that of patients with catabasis UC,and the proportion of MDSCs,WBC count and PLT count increased significantly in patients with active UC(P<0.05).MDSCs positively correlatd with WBC count and PLT count (P<0.05) while negatively correlated with CD 4+CD25 +Treg(P<0.05) in UC patients.Conclusion The level of MDSCs in peripheral blood increases significantly in UC patients ,and it correlates with activity of UC .The detection of MDSCs may benefit understanding the pathogenesis and auxiliary diagnosis of UC .