CAJ | 학술논문

目的：探讨大肠癌细胞中人类白细胞分化抗原133（ CD133）与八聚体结合转录因子4（ OCT4）的表达情况及其相关性。方法采用磁珠细胞分离技术分选出人结肠癌细胞系的CD133＋细胞和CD133-细胞，检测两种细胞中OCT4的表达。分别改变CD133＋和CD133-细胞中OCT4的表达，观察其对CD133的表达的影响。采用肿瘤球形成实验检验肿瘤细胞干性生物学行为的改变。结果 CD133＋细胞中OCT4呈现高表达， CD133-细胞中OCT4基本无表达。 CD133＋细胞对5-氟尿嘧啶（5-FU ）高度抵抗，并且更容易在无血清培养基中形成肿瘤球。干扰OCT4的表达可以降低CD133＋细胞中CD133的表达水平，增加对5-FU的敏感性，并且减少肿瘤球形成能力。过表达OCT4增加CD133-细胞CD133的表达水平，降低对5-FU的敏感性，并且减少肿瘤球形成能力。结论 OCT4与CD133在大肠癌细胞中均有表达，OCT4对CD133有直接调控作用。
목적：탐토대장암세포중인류백세포분화항원133（ CD133）여팔취체결합전록인자4（ OCT4）적표체정황급기상관성。방법채용자주세포분리기술분선출인결장암세포계적CD133＋세포화CD133-세포，검측량충세포중OCT4적표체。분별개변CD133＋화CD133-세포중OCT4적표체，관찰기대CD133적표체적영향。채용종류구형성실험검험종류세포간성생물학행위적개변。결과 CD133＋세포중OCT4정현고표체， CD133-세포중OCT4기본무표체。 CD133＋세포대5-불뇨밀정（5-FU ）고도저항，병차경용역재무혈청배양기중형성종류구。간우OCT4적표체가이강저CD133＋세포중CD133적표체수평，증가대5-FU적민감성，병차감소종류구형성능력。과표체OCT4증가CD133-세포CD133적표체수평，강저대5-FU적민감성，병차감소종류구형성능력。결론 OCT4여CD133재대장암세포중균유표체，OCT4대CD133유직접조공작용。
Objective To explore the expressions of human leukocyte differentiation antigen 133 ( CD133 ) and octamer binding transcription factor 4 ( OCT4 ) in colon cancer cells and their correlation .Methods CD133 +cells and CD133-cells were separated by immunomagnetic separation technology .Their OCT4 expressions were detected and changed,and the effect of OCT4 expressions on CD133 expression was observed .Tumor sphere formation assay was employed to test the cancer stem cell phenotype .Results CD133+ cells showed a high expression of OCT 4, and CD133-cells showed no expression of OCT 4.CD133+cells were of high resistance to 5-fluorouracil (5-FU),and were easier to form spheres in the serum-free medium.Inhibiting the OCT4 expression reduced the CD 133 expression in CD133+cells,increased the 5-FU sensitivity,and reduced the tumor sphere formation ability .Over-expression of OCT4 increased CD133 expression in CD133-cells,decreased 5-FU resistance,and reduced tumor sphere formation ability . Conclusion Both OCT4 and CD133 are expressed in colon cancer cells,OCT4 may play a role on regulating CD133 directly.