CAJ | 학술논문

本实验建立了高效液相色谱法（HPLC）测定饲料中癸氧喹酯含量的方法。该方法采用C18色谱柱（250 mm×4.6 mm，粒径5μm），以0.025 mol/L氯化钙溶液+甲醇（15∶85，V/V）为流动相，激发波长为326 nm，发射波长为384 nm，流速为1.0 mL/min，外标法定量。结果表明，该方法最低检测限为0.5 mg/kg，癸氧喹酯的浓度为0.02～2.0 mg/L时，峰面积与浓度线性关系良好（r=0.99994），平均回收率在80%以上，变异系数小于10%，稳定性、重现性好，可用于饲料中癸氧喹酯的含量测定。
본실험건립료고효액상색보법（HPLC）측정사료중계양규지함량적방법。해방법채용C18색보주（250 mm×4.6 mm，립경5μm），이0.025 mol/L록화개용액+갑순（15∶85，V/V）위류동상，격발파장위326 nm，발사파장위384 nm，류속위1.0 mL/min，외표법정량。결과표명，해방법최저검측한위0.5 mg/kg，계양규지적농도위0.02～2.0 mg/L시，봉면적여농도선성관계량호（r=0.99994），평균회수솔재80%이상，변이계수소우10%，은정성、중현성호，가용우사료중계양규지적함량측정。
This experiment was conducted to study the method for high performance liquid chromatographic (HPLC) for determination of decoquinate in feeds by HPLC method.A C18 column (250 mm×4.6 mm,5μm)was used with the mo-bile phase of 0.025 mol/L calcium chloride-methanol (15∶85,V/V)at the excitation wavelength of 326 nm and emission wavelength of 384 nm with a flow rate of 1.0 mL/min.The quantification was carried out by the external standard method. The limit of quantitation(LOQ)was 0.5 mg/kg.The calibration curves had good linear within 0.02 ~ 2.0 mg/L(r=0.99994). The average recovery was above 80%.The variation coefficient was less than 10%.The results showed that the method had advantages of good stability and reproducibility,and could be used for the determination of decoquinate in feed.