CAJ | 학술논문

本实验以山羊瘤胃液为样品，建立了反相高效液相色谱（RP-HPLC）测定乙酸、丙酸和丁酸等挥发性脂肪酸含量的方法。结果表明：甲醇与0.01 mol/L磷酸二氢钠缓冲液（pH=2.6）比例为28∶72时可很好地分离乙酸、丙酸和丁酸，回收率均大于83%，相对标准偏差（RSD）均小于0.67%。
본실험이산양류위액위양품，건립료반상고효액상색보（RP-HPLC）측정을산、병산화정산등휘발성지방산함량적방법。결과표명：갑순여0.01 mol/L린산이경납완충액（pH=2.6）비례위28∶72시가흔호지분리을산、병산화정산，회수솔균대우83%，상대표준편차（RSD）균소우0.67%。
Goat rumen fluid was used to establish a method of determination acetic,propionic and butyric acid by us-ing reversed phase high performance liquid chromatography(RP-HPLC).The results showed that:acetic,propionic and bu-tyric acid could be well separated by the methanol mixed with sodium dihydrogen phosphate buffer (0.01 mol/L pH=2.6) by 28∶72,and the recoveries of the three volatile fatty acids (VFA)were larger than 83%,relative standard deviation (RSD) were less than 0.67%.