中国饲料
中國飼料
중국사료
CHINA FEED
2014年
13期
22-24
,共3页
何海燕%骆成锐%黎风华%陆世泽%覃拥灵
何海燕%駱成銳%黎風華%陸世澤%覃擁靈
하해연%락성예%려풍화%륙세택%담옹령
果胶酶%蚕沙%正交试验%条件优化
果膠酶%蠶沙%正交試驗%條件優化
과효매%잠사%정교시험%조건우화
pectinases%silkworm faeces%orthogonal experiment%optimization of conditions
本试验以蚕沙为原料，利用米曲霉发酵生产果胶酶，通过单因素试验和正交设计试验对产酶条件进行了优化，试验结果显示：在培养基初始pH为自然，蚕沙添加量为4%，接种量4%，发酵温度36℃，发酵时间96 h的培养条件下，测得酶活为131.3 U/mL，比初始培养条件的酶活力提高了229%。
本試驗以蠶沙為原料，利用米麯黴髮酵生產果膠酶，通過單因素試驗和正交設計試驗對產酶條件進行瞭優化，試驗結果顯示：在培養基初始pH為自然，蠶沙添加量為4%，接種量4%，髮酵溫度36℃，髮酵時間96 h的培養條件下，測得酶活為131.3 U/mL，比初始培養條件的酶活力提高瞭229%。
본시험이잠사위원료，이용미곡매발효생산과효매，통과단인소시험화정교설계시험대산매조건진행료우화，시험결과현시：재배양기초시pH위자연，잠사첨가량위4%，접충량4%，발효온도36℃，발효시간96 h적배양조건하，측득매활위131.3 U/mL，비초시배양조건적매활력제고료229%。
In this experiment,silkworm faeces was used as fermentation raw material for pectinase production with Aspergillus oryzae.Based on the single factor and orthogonal design experiments,the optimal combinations were confirmed as follow:initial pH of medium was nature,the silkworm faeces content was 4%,inoculum concentration was 4%,which was culture on the shake bed at 36℃for about 96 h,and under the optimized conditions,the enzyme activity was 131.3 U/mL, 229%higher than that of the initial fermentation conditions.
