CAJ | 학술논문

试验采用微波辅助酸法提取菜籽粕多糖。在正交试验的基础上通过Box-Behnken中心组合进行三因素三水平试验，对多糖的提取工艺进行优化。结果表明：最佳提取工艺为盐酸浓度0.3 mol/L、微波功率200 W、微波时间8 min、料液比1∶26（g/mL）、微波温度63℃，在此条件下，菜籽粕多糖的提取率达3.1763%。多糖的性质研究结果表明：菜籽粕多糖有一定的清除羟自由基能力，最大清除率达85.79%；Fe3+和Co2+对多糖稳定性影响较大，在配制饲料时应避免直接接触。
시험채용미파보조산법제취채자박다당。재정교시험적기출상통과Box-Behnken중심조합진행삼인소삼수평시험，대다당적제취공예진행우화。결과표명：최가제취공예위염산농도0.3 mol/L、미파공솔200 W、미파시간8 min、료액비1∶26（g/mL）、미파온도63℃，재차조건하，채자박다당적제취솔체3.1763%。다당적성질연구결과표명：채자박다당유일정적청제간자유기능력，최대청제솔체85.79%；Fe3+화Co2+대다당은정성영향교대，재배제사료시응피면직접접촉。
The microwave-assisted extraction technology of polysaccharides from rapeseed cake by acid method was studied.The extraction technologies were optimized using 3 factors and 3 levels Box-Behnken central combination design based on the orthogonal test.The results showed that:the optimum conditions were the concentration of hydrochloric acid 0.3 mol/L,microwave power 200 W,microwave treatment 8 min,microwave temperature 63 ℃,ratio of solid of solvent 1∶26(g/mL).The extraction rate of polysaccharides was 3.1763%under optimal extraction conditions.The results of proper-ties experiment showed that rapeseed cake polysaccharides had certain activities to scavenge ·OH and the highest scav-enging ratio was 85.79%;Fe3+and Co2+ had great effect on the stability of polysaccharide,and should be avoided during feed preparation.