CAJ | 학술논문

探讨了HPLC测定当归药材中阿魏酸含量的方法。以乙腈-0.085％磷酸（17：83）为流动相；检测波长为316 nm，取样量为0.2 g，70％甲醇回流提取30 min，80℃的水浴加热的浓缩温度。结果表明：阿魏酸在进样2.4～38.4μg· mL-1范围内呈良好的线性关系，平均回收率为103.1463％， RSD为0.4547％。高效液相色谱法可用于当归药材中阿魏酸含量的测定，方法简单准确，灵敏度高。
탐토료HPLC측정당귀약재중아위산함량적방법。이을정-0.085％린산（17：83）위류동상；검측파장위316 nm，취양량위0.2 g，70％갑순회류제취30 min，80℃적수욕가열적농축온도。결과표명：아위산재진양2.4～38.4μg· mL-1범위내정량호적선성관계，평균회수솔위103.1463％， RSD위0.4547％。고효액상색보법가용우당귀약재중아위산함량적측정，방법간단준학，령민도고。
The method of determining the content of ferulic acid in angelica sinensis by HPLC was studied.With acetonitrile-0.085%phosphate (17:83) as mobile phase, the wavelength was detected of 316 nm, sampling quantity was 0.2 g, 70% methanol circumfluence extraction 30 min, the concentration of the water bath heating temperature of 80 ℃.Ferulic acid in the sample range 2.4 ~38.4 μg/mL had good linear relationship , the average recovery was 103.1463%, and RSD was 0.4547%.High performance liquid chromatography ( HPLC ) method can be used for the determination of ferulic acid content of angelica sinensis medicinal material , and the method was simple and accurate , and high sensitivity.